银柴胡组织培养与植株再生体系的建立  

Establishment of Tissue Culture and Plant Regeneration System of Stellaria dichotoma

作　　者：姚宁 马晓蓉 高建中 乔亚蕊 徐婷婷[1] 梁文裕[1] Yao Ning;Ma Xiaorong;Gao Jianzhong;Qiao Yarui;Xu Tingting;Liang Wengyu(School of Life Sciences,Ningxia University,Yinchuan,750021)

机构地区：[1]宁夏大学生命科学学院,银川750021

出　　处：《分子植物育种》2024年第17期5795-5802,共8页Molecular Plant Breeding

基　　金：宁夏回族自治区科技创新团队建设项目(NXKJT2019002)资助。

摘　　要：银柴胡是著名的药用植物。以MS为基本培养基,银柴胡种子无菌培养获得外植体,通过添加不同浓度的6-苄基氨基嘌呤(6-BA)、萘乙酸(NAA)、6-呋喃氨基嘌呤(KT)、吲哚丁酸(IBA)等植物激素探究对银柴胡不定芽、不定根诱导的最佳激素配比,并进行炼苗与移栽试验。结果表明,银柴胡种子用15%NaClO处理10 min消毒效果最好,污染率仅为3.37%,萌发率为97.15%;不定芽诱导的最佳培养基为:MS+6-BA 1.5 mg/L+NAA 0 mg/L,诱导率为100%;诱导不定芽生根的最佳培养基为:1/2MS+6-BA 0.3 mg/L+IBA 0.5 mg/L,生根率可达90%;以组培瓶盖半开的方式炼苗5 d后移栽至基质土中,成活率达到100%。采用直接器官发生途径,成功建立了银柴胡组织培养与植株再生体系,研究结果为银柴胡良种繁育和遗传改良提供参考依据。Stellaria dichotoma is a famous medicinal plant.Using MS as the basic medium,the explants were obtained by aseptic culture of S.dichotoma.By adding different concentrations of plant hormones such as 6-benzylaminopurine(6-BA),naphthylacetic acid(NAA),6-furfuryl aminopurine(KT),indole butyric acid(IBA),the optimal hormone ratio for the induction of adventitious buds and roots of S.dichotoma was explored,and the seedling refining and transplanting experiments were carried out.The results showed that the disinfection effect of S.dichotoma treated with 15% NaCl for 10 min was the best,the pollution rate was only 3.37%,and the germination rate was 97.15%;the best medium for adventitious bud induction was MS+6-BA 1.5 mg/L+NAA 0 mg/L,and the induction rate was 100%;the best medium for inducing adventitious bud rooting was 1/2 MS+6-BA 0.3 mg/L+IBA 0.5 mg/L,and the rooting rate could reach 90%;the seedlings were refined by half opening the cap of tissue culture bottle for 5 days and transplanted into matrix soil,and the survival rate was 100%.Using direct organogenesis,a system of tissue culture and plant regeneration of S.dichotoma was successfully established.These results will laid a foundation for the elite breeding and genetic improvement of S.dichotoma.

关 键 词：银柴胡 带节茎段 组织培养 植株再生体系 

分 类 号：S567.79[农业科学—中草药栽培]