电针刺激启动穴位效应促进骨骼肌修复的机制研究  

Study on the mechanism of electroacupuncture stimulation to activate the acupoint effect and promote skeletal muscle repair

作　　者：黄于婷 廖军[1] 饶天予 刘科志 林佳[1] 林煜也 曾楚凡 张国俊 张夏 童秀冰 张靖宇 阚宇 方燕平 景向红[2] 王晅[1] HUANG Yuting;LIAO Jun;RAO Tianyu;LIU Kezhi;LIN Jia;LIN Yuye;ZENG Chufan;ZHANG Guojun;ZHANG Xia;TONG Xiubing;ZHANG Jingyu;KAN Yu;FANG Yanping;JING Xianghong;WANG Xuan(Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;Institute of Acupuncture and Moxibustion,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]福建中医药大学,福州350122 [2]中国中医科学院针灸研究所

出　　处：《北京中医药大学学报》2024年第8期1160-1170,共11页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(No.82204908,No.82074181,No.82374202);福建省科技厅项目(No.2023J01151,No.2022J01874);福建中医药大学校管课题(No.X2019010-人才专项)。

摘　　要：目的探讨电针刺激启动穴位效应激活肌卫星细胞促进骨骼肌急性损伤修复的机制。方法将45只SPF级2月龄SD大鼠随意分为空白组、模型组、电针组、色甘酸钠组、色甘酸钠电针组,每组9只。除空白组外,各组大鼠采用自制打击器建立右侧腓肠肌急性钝挫伤模型。色甘酸钠模型组、色甘酸钠电针组在右侧“承山”“阳陵泉”注射20μL色甘酸钠,1次/d,干预7 d。电针组及色甘酸钠电针组大鼠于右侧“承山”“阳陵泉”行电针干预,予疏密波(2 Hz/100 Hz),每次30 min,1次/d,干预7 d。采用动物步态分析仪记录大鼠步态变化;HE染色法观察大鼠右侧腓肠肌形态学变化;甲苯胺蓝染色观察“承山”局部皮肤肌肉肥大细胞聚集及脱颗粒状态变化。免疫荧光法检测大鼠右侧腓肠肌的肌卫星细胞配对盒基因7(Pax7)、成肌分化因子(MyoD),以及穴区皮肤肥大细胞类胰蛋白酶、5-羟色胺(5-HT)阳性表达;免疫组织化学染色观察右侧腓肠肌巨噬细胞标志物CD68和CD206的阳性表达。结果与空白组比较,模型组和色甘酸钠组右后肢摆动时间增加,步幅减小,HE染色可见炎性细胞浸润、肌细胞肿大、变性坏死,“承山”局部皮肤肥大细胞脱颗粒率增加,肥大细胞类胰蛋白酶、5-HT、Pax7、MyoD、CD68、CD206表达增加(P<0.05);与模型组比较,电针组和色甘酸钠电针组右后肢摆动时间减少,步幅增大,HE染色见炎性细胞浸润减轻,肌细胞大小较一致,排列整齐;电针组肥大细胞脱颗粒率明显增加,肥大细胞类胰蛋白酶、5-HT、Pax7、MyoD、CD206表达增加(P<0.05),CD68表达减少(P<0.05);与色甘酸钠电针组比较,电针组肥大细胞脱颗粒率增加,肥大细胞类胰蛋白酶、5-HT、Pax7、MyoD、CD206表达增加(P<0.05),CD68表达减少(P<0.05)。结论电针“承山”“阳陵泉”可刺激穴位局部,诱导肥大细胞脱颗粒,调控巨噬细胞表达,进而激活肌卫星细胞发挥修复骨骼�Objective To investigate the mechanism of inducing macrophage polarization induced by acupoint effect of electroacupuncture to promote the repair of acute skeletal muscle injury.Methods 45 SD rats were randomly divided into blank group,model group,electroacupuncture group(EA group),sodium chrominate group(DSCG group)and electroacupuncture+sodium chrominate group(hereinafter referred to as EA+DSCG group),with 9 rats in each group.The rats in the EA group and the EA+DSCG group were subjected to EA intervention at the right"Chengshan"(BL57)and"Yanglingquan"(GB34),with a frequency of 2 Hz/100 Hz.The gait changes of rats were recorded by animal gait analyzer.The morphological changes of the right gastrocnemius were observed by HE staining.The changes of mast cell aggregation and degranulation in local skin muscles of"chengshan"point were observed by toluidine blue staining.The expressions of Pax7,MyoD and skin mast cells and 5-HT in the right gastrocnemius were detected by immunofluorescence method.The positive expressions of CD68 and CD206 in right gastrocnemius macrophage was observed by immunohistochemical staining.Results Compared with blank group,the wiggle time of the right hind leg in model group and DSCG group increased,stride length decreased,HE staining showed inflammatory cell infiltration,myocyte enlargement,degeneration and necrosis.The degranulation rate of local skin mast cells in"Chengshan"(BL57)area increased,and the expressions of mast cell tryptase,5-HT,Pax7,MyoD,CD68 and CD206 increased(P<0.05).Compared with model group,the wiggle time of the right hind leg in EA group and EA+DSCG group decreased,stride length increased,HE staining showed that inflammatory cell infiltration was reduced,muscle cells were uniform in size and arranged neatly.Mast cell degranulation rate increased significantly in EA group,and the expressions of mast cell tryptase,5-HT,Pax7,MyoD and CD206 increased(P<0.05),while CD68 expression decreased(P<0.05).Compared with EA+DSCG group,the degranulation rate of mast cells and the e

关 键 词：电针 穴位效应 巨噬细胞 肌卫星细胞 

分 类 号：R246.2[医药卫生—针灸推拿学]