喹乙醇对体外卵母细胞及胚胎发育的影响  

Effects of olaquindox on oocyte and embryo development in vitro

作　　者：王巨龙 黎婷 张海燕[1] 朱广双[1] 刘宽辉 WANG Julong;LI Ting;ZHANG Haiyan;ZHU Guangshuang;LIU Kuanhui(College of Food and Biological Engineering,Wuhu Institute of Technology,Wuhu 241003,China)

机构地区：[1]芜湖职业技术学院食品与生物工程学院,安徽芜湖241003

出　　处：《商丘师范学院学报》2024年第9期27-30,共4页Journal of Shangqiu Normal University

基　　金：安徽省高校自然科学研究项目(2022AH052193);安徽省高校青年人才支持计划(gxyq2022203);安徽省教育厅自然科学项目(KJ2021A1323);芜湖职业技术学院2021年校级中青年骨干教师项目;芜湖职业技术学院2021年校级优秀拔尖人才项目。

摘　　要：喹乙醇(Olaquindox,OLA)作为有效的抗菌药剂,广泛应用于畜禽养殖业.然而,喹乙醇是否对体外卵母细胞及胚胎发育有影响尚不清楚.本实验采用不同浓度喹乙醇(0.1 mM,0.5 mM,1.0 mM,2.0 mM,5.0 mM)对体外获取生发泡(Germinal vesicle,GV)期卵母细胞体外培养,观察卵母细胞的体外成熟率.其次,对实验组体外成熟卵母细胞进行激活发育,统计卵细胞激活率.最后,使用喹乙醇处理体外受精卵观察胚胎发育情况,观察胚胎各阶段发育率并通过荧光定量PCR囊胚期OCT4(Octamer-binding transcription factor 4,OCT4)、CDX2(Caudal type homeobox 2,CDX2)基因表达水平.实验结果显示,1.0 mM、2.0 mM、5.0 mM OLA处理GV期卵母细胞显著阻碍卵母细胞成熟(P<0.05);5.0 mM处理下卵母细胞激活率显著降低(P<0.01).同时,高剂量的喹乙醇处理下,早期胚胎囊胚发育受阻,相关发育基因OCT4、CDX2 mRNA表达水平呈显著下降.As an effective antibacterial agent,olaquindox(OLA)has been widely used in livestock and poultry industry.However,it is unclear whether olaquindox has an effect on oocyte and embryo development in vitro.In this study,GV stage oocytes were exposed to different concentrations of olaquindox(0.1,0.5,1.0,2.0,5.0 mM).Observation of oocyte maturation rate after a period of in vitro culture.Secondly,the matured oocytes in vitro were activated and developed,and the activation rate of oocytes was calculated.Finally,the in vitro fertilized eggs were treated with oquindox to observe the embryonic development,the developmental rate of embryos at each stage and the expression levels of OCT4 and CDX2 genes at the blastocyst stage by fluorescent quantitative PCR.The experimental results show that 1.0 mM,2.0 mM,5.0 mM OLA treatment of GV-stage oocytes significantly blocked oocyte maturation(P<0.05).The activation rate of oocytes under 5.0 mM OLA treatment decreased significantly(P<0.01).Meanwhile,the experiment showed that high-dose OLA impaired early embryo development and the mRNA expression levels of related developmental genes OCT4 and CDX2 decreased significantly.

关 键 词：喹乙醇 卵母细胞发育 胚胎发育 胚胎质量 

分 类 号：Q31[生物学—遗传学]