甘肃省陇南市核桃枝枯病菌Alternaria alternate的生物学特性  

Biological Characteristics of Alternaria alternate Causing Walnut Branch Blight in Longnan City,Gansu Province

作　　者：邓小芸 高孟春 杨成德 DENG Xiao-yun;GAO Meng-chun;YANG Cheng-de(Bailongjiang Forestry Management Bureau of Gansu Province,Lanzhou,Gansu 730000;College of Plant Protection of Gansu Agricultural University,Lanzhou,Gansu 730070)

机构地区：[1]白龙江林业保护中心,甘肃兰州730000 [2]甘肃农业大学植物保护学院,甘肃兰州730070

出　　处：《安徽农业科学》2024年第17期133-137,共5页Journal of Anhui Agricultural Sciences

摘　　要：以核桃枝枯病菌(Alternaria alternate)为对象,研究温度、碳源、氮源、光照、培养基和pH等对菌丝生长、产孢量和孢子萌发的影响。结果表明,pH 7和全黑暗有利于核桃枝枯病菌的菌丝生长、产孢和孢子萌发;25℃有利于菌丝生长,25~30℃有利于产孢,20~30℃有利于孢子萌发;最适生长的碳源为可溶性淀粉,氮源为酵母浸粉、KNO_(3)和蛋白胨,最适产孢的碳源和氮源分别为山梨醇和KNO_(3),最适孢子萌发的碳源和氮源分别为乳糖和NH_(4)NO_(3);PSA、OA和PDA培养基适合菌丝生长,LB培养基适合产孢,OA培养基适合孢子萌发。该研究结果为明确核桃枝枯病的发生规律提供理论依据,也为综合防治措施的制定提供参考。The effects of temperature,carbon source,nitrogen source,light,medium and pH on mycelial growth,sporulation and spore germination rate were studied.The results showed that pH 7 and total darkness were conducive to the mycelial growth,sporulation and spore germination of Alternaria alternata;25℃was optimum to mycelial growth,25-30℃was optimum to sporulation,and 20-30℃was optimum to spore germination;the optimum carbon source for mycelial growth was soluble starch,the nitrogen source was yeast extract,KNO_(3)and peptone,the optimum carbon source and nitrogen source for sporulation were sorbitol and KNO_(3),respectively,and the optimum carbon source and nitrogen source for spore germination were lactose and NH_(4)NO_(3),respectively;PSA,OA and PDA medium were suitable for mycelial growth,LB medium was suitable for sporulation,and OA medium was suitable for spore germination.The results provide a theoretical basis for clarifying the occurrence rules of walnut branch blight,and also provide a reference for the comprehensive control measures.

关 键 词：交链格孢菌 生物学特性 测定 

分 类 号：S436.621.1[农业科学—农业昆虫与害虫防治]