根际促生菌对茄子、南瓜、辣椒穴盘苗胚轴和根际微生物多样性的影响  

Effects of Plant Growth-promoting Rhizobacteria on Microbial Diversity in Hypocotyl and Rhizosphere of Plug Seedlings of Eggplant,Pumpkin and Pepper

作　　者：张锋[1,2] 张顺顺 尚庆茂 ZHANG Feng;ZHANG Shunshun;SHANG Qingmao(National Nanfan Research Institute of Chinese Academy of Agricultural Sciences,Sanya 572024,China;Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences/State Key Laboratory of Vegetable Biobreeding,Beijing 100081,China)

机构地区：[1]三亚中国农业科学院国家南繁研究院,海南三亚572024 [2]中国农业科学院蔬菜花卉研究所/蔬菜生物育种全国重点实验室,北京100081

出　　处：《蔬菜》2024年第9期39-49,共11页Vegetables

基　　金：三亚市农业农村局“院地联合”攻关项目(YDLH05);海南省重大科技计划项目(ZDKJ2021005);中央引导地方区域创新体系建设项目(XZ202202YD0002C-10);财政部和农业农村部国家现代农业产业技术体系资助(CARS-23-B10);中央级公益性科研院所基本科研业务费专项(IVF-BRF2023006)。

摘　　要：为探究根际促生菌对蔬菜幼苗胚轴和根际微生物群落多样性的影响,以茄子品种“京茄9号”、南瓜品种“超人”、辣椒品种“京旋1号”和解淀粉芽孢杆菌(L-S60、LPL-117、L-H15)为试验材料,利用Illumina NovaSeq高通量测序技术和生物信息学分析方法,分析了在根际接种解淀粉芽孢杆菌后蔬菜穴盘苗胚轴和根际微生物的多样性。结果表明:接种3个根际促生菌均能改变蔬菜幼苗胚轴和根际细菌特征序列的种类。α多样性分析表明,根际接种L-S60后降低了南瓜胚轴微生物Shannon指数和辣椒根际微生物的Chao1指数、Obserbed otus指数、Shannon指数;根际接种LPL-117后提高了茄子胚轴微生物的Chao1指数、Obserbed otus指数,降低了辣椒根际微生物的Chao1指数、Obserbed otus指数、Shannon指数;根际接种L-H15后降低了辣椒根际微生物的Chao1指数、Obserbed otus指数、Shannon指数。β多样性分析表明,在主成分1(PC1)上3种蔬菜接种3个根际促生菌后的胚轴和根际的细菌群落多样性均分别有明显区分。说明根际接种根际促生菌可影响蔬菜穴盘苗根际微生物群落的结构和组成,3株解淀粉芽胞杆菌可施用于蔬菜穴盘苗根际,用于改善幼苗根际的微生态环境。In order to explore the effects of plant growth-promoting rhizobacteria(PGPR)on the microbial community diversity in hypocotyl and rhizosphere of vegetable plug seedlings,eggplant variety of'Jingqie No.9',pumpkin variety of'Chaoren',pepper variety of'Jingxuan No.1'and three strains of Bacillus amyloliquefaciens L-S60,LPL-117 and L-H15 were used as experimental materials.The microbial community diversity in hypocotyl and rhizosphere of vegetable plug seedlings after inoculation with Bacillus amyloliquefaciens in rhizosphere was analyzed by using Illumina NovaSeq high-throughput sequencing technology and bioinformatics analysis method.The results showed that inoculation of three strains of Bacillus amyloliquefaciens could change the types of hypocotyl and rhizosphere bacterial characteristic sequences of vegetable plug seedlings.α-diversity analysis results showed that Shannon index of pumpkin hypocotyls microorganisms and Chao1 index,Obserbed otus index and Shannon index of pepper rhizospheric microorganisms decreased after inoculating L-S60 in rhizosphere.Chao1 index,Obserbed otus index of hypocotyl microorganisms of eggplant increased after inoculating LPL-117 in rhizosphere,but the Chao1 index,Obserbed otus index and Shannon index of pepper rhizospheric microorganisms decreased.Chao1 index,Obserbed otus index and Shannon index of pepper rhizospheric microorganisms decreased after inoculating L-H15 in rhizosphere.β-diversity analysis results showed that the diversity of bacterial communities in hypocotyl and rhizosphere of three vegetables inoculated with three strains of Bacillus amyloliquefaciens on principal component 1(PC1)was obviously distinguished.It indicated that inoculation of PGPR could affect the structure and composition of microbial community in rhizosphere of vegetable plug seedlings,and three strains of Bacillus amyloliquefaciens could be applied to the rhizosphere of vegetable plug seedlings to improve the microecological environment of rhizosphere of seedlings.

关 键 词：解淀粉芽孢杆菌 茄子 南瓜 辣椒 微生物多样性 高通量测序技术 引物序列 物种注释 

分 类 号：S641.1[农业科学—蔬菜学] S642.1[农业科学—园艺学] S641.3