Variations in diversity,composition,and species interactions of soil microbial community in response to increased N deposition and precipitation intensity in a temperate grassland  

作　　者：Shuyan Cui Yushan Xiao Yu Zhou Pengfeng Wu Liqiang Cui Guo Zheng 

机构地区：[1]College of Life Science,Shenyang Normal University,Shenyang 110034,China [2]School of Environmental Science and Engineering,Yancheng Institute of Technology,Yancheng 224051,China

出　　处：《Ecological Processes》2023年第1期495-506,共12页生态过程（英文）

基　　金：supported by the National Natural Science Foundation of China(NSFC-31970410);Liaoning Revitalization Talents Program(XLYC2002083);Liaoning Province Science and Technology Plan Project(2018103004);Department of Science and Technology of Liaoning Province(2022JH5/10400111).

摘　　要：Background Global climate change has resulted in precipitation regimes exhibiting an increasing trend in rainfall intensity but a reduction in frequency.In addition,nitrogen(N)deposition occurs simultaneously in arid and semi-arid regions.Microbial biomass,diversity,composition,and species interactions are key determinants of ecological functions.We examined the effects of changes in precipitation intensity and N addition on the soil bacterial and fungal communities in a semi-arid grassland in Inner Mongolia,China.Methods The microbial biomass(bacterial PLFAs and fungal PLFAs)was determined through phospholipid fatty acid(PLFA)analysis,and microbial diversity(Shannon index and evenness index)was determined with high-throughput sequencing(16S and ITS).Species interactions were determined using a molecular ecological network analysis.The relationships between microbial community(bacterial community and fungal community)and environmental variables were examined by Mantel tests.Results We found that N addition decreased fungal PLFA under moderate,high,and extreme precipitation intensity treatments and increased fungal community complexity under the high precipitation intensity treatment.Furthermore,N addition increased bacterial diversity under moderate and high precipitation intensity treatments.N addition caused greater environmental stress to the fungal community,which was dominated by deterministic processes.Conclusions The effects of N deposition on soil bacterial and fungal communities were altered by precipitation intensity.The changes in soil bacterial and fungal communities were different,implying that composition and functional traits adapt differently to projected global changes at a regional scale.

关 键 词：Microbial community composition Phospholipid fatty acid High-throughput sequencing Precipitation intensity N deposition 

分 类 号：S812.2[农业科学—草业科学]