Ultrasound-assisted isolation:A new method to isolate stromal vascular fraction  

作　　者：Yiming Gao Xiaojie Zhang Poh-Ching Tan Yun Xie Peiqi Zhang Tianyu Zhang Qingfeng Li Shuangbai Zhou 

机构地区：[1]Department of Plastic and Reconstructive Surgery,Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,200011,China [2]Department of Burns and Plastic Surgery,Shanghai Changzheng Hospital,Shanghai,200003,China [3]College of Life Sciences,Shanghai Normal University,Shanghai,200233,China

出　　处：《Chinese Journal of Plastic and Reconstructive Surgery》2024年第1期8-15,共8页

基　　金：supported by the National Natural Science Foundation of China(grant nos.81971848 and 82272287);Shanghai Municipal Key Clinical Specialty(grant no,shslczdzk00901);Clinical Research Plan of SHDC(rant nos.SHDC2020CR1019B and SHC2020CR402);Innovative Research Team of High-Level Local Universities in Shanghai(grant no.SSMU-ZDCX20180700);Shanghai Clinical Research Center of Plastic and Reconstructive Surgery supported by the Science and Technology Commission of Shanghai Municipality(grant no.22MC1940300).

摘　　要：Background:The stromal vascular fraction(SVF),a cluster of stem and progenitor cells isolated from adipose tissue,holds significant promise for application in regenerative medicine.However,the existing methods for SVF isolation are time-consuming and expensive.Thus,in this study,we explored a new method of SVF extrac-tion-ultrasound-assisted SVF isolation(USASI)-and compared the viability and characteristics of SVF isolated using different methods.Methods:SVF extraction methods using different combinations of ultrasound power,ultrasound time,collagenase dosage,and collagenase digestion time were compared with those of the control group(collagenase digestion method).The cell yield and vitality of the SVF were evaluated via cell counting and trypan blue staining.The cell components and immunophenotypes of freshly isolated SVF were analyzed using flow cytometry.The prolifer-ative capacity and differentiation potential of the SVF were also identified.Results:Ultrasonication at 95 W-20 kHz for 30 s followed by digestion with 0.15%collagenase for 30 min was identified as the most suitable parameter for the USASI method in isolating SVF,as recommended based on the evaluation of various tested conditions.The USASI method significantly reduced the collagenase dosage and shortened the digestion time.Compared to the collagenase digestion method,the USASI method had a higher cell yield and cell viability,with no adverse effects on cell components,proliferative capacity,or multipotential differentiation capacity.Conclusion:With reduced processing time,lower collagenase dosage,and increased cell yield without impairing the viability and characteristics of SVF,USASI holds the potential to emerge as a time-saving and cost-effective method for future clinical applications.

关 键 词：Stromal vascular fractions SVF isolation Mechanical force ULTRASOUND 

分 类 号：R622[医药卫生—整形外科]