Exploration of mangrove‑associated actinobacteria from South Andaman Islands,India  

作　　者：Balakrishnan Meena Lawrance Anburajan Munir Ahamed Johnthini Nambali Valsalan Vinithkumar Gopal Dharani 

机构地区：[1]Atal Centre for Ocean Science and Technology for Islands,National Institute of Ocean Technology,Ministry of Earth Sciences,Government of India,Dollygunj,Port Blair 744103,Andaman and Nicobar Islands,India [2]Marine Biotechnology Division,Ocean Science and Technology for Islands Group,National Institute of Ocean Technology,Ministry of Earth Sciences,Government of India,Chennai 600100,Tamil Nadu,India [3]Department of Marine Science,Bharathidasan University,Tiruchirappalli 620024,Tamil Nadu,India

出　　处：《Systems Microbiology and Biomanufacturing》2023年第4期702-718,共17页系统微生物学与生物制造（英文）

摘　　要：In this study,a total of 1125 actinobacteria were isolated from the selected mangrove species:Avicennia marina,Rhizopora mucronata and Ceriops tagal from three study stations viz.,Minnie Bay,Carbyn’s Cove and Burmanallah.Among these three stations,the highest number of actinobacteria was recorded in Carbyn’s Cove(64.97%),followed by(25.51%)at Burmanallah and the minimum of(9.51%)was recorded in Minnie Bay.Maximum number of actinobacteria was recorded from Ceriops tagal(40.44%)than the other selected mangrove species Avicennia marina(34.13%)and Rhizopora mucronata(25.42%).Among the 1,125 mangrove-associated actinobacteria,103 morphologically different isolates from the Minnie Bay station was selected for the further characterization studies.In antibacterial assay,30.11%of the isolates revealed inhibitory activ-ity against all tested clinical pathogens and 65%isolates displayed inhibitory activity against minimum of 04 tested clinical pathogens.Growth survival studies of the actinobacterial isolates also accomplished to withstand in varied NaCl and pH levels.Of 103 isolates,all were found to synthesize gelatinase enzyme,73 isolates demonstrated amylolytic activity,38 isolates exhibited proteolytic and 63 isolates displayed urease activity.Interestingly,56 isolates exhibited excellent DNase activity and 71 isolates revealed positive for l-asparaginase production.To our recognition,11 isolates exhibited constructive results in the production of 06 extracellular enzymes of industrial importance.Of 103 isolates,48 isolates were confirmed by molecular level identification.Based on the phylogenetic analysis,the isolates were categorized under the genera:Strep-tomyces,Nocardiopsis,Salinispora and Actinomadura.

关 键 词：MANGROVE ACTINOBACTERIA Avicennia marina Rhizopora mucronata Ceriops tagal 

分 类 号：Q93[生物学—微生物学]