Improvement of lycopene biosynthesis in waaC and waaF mutants of Escherichia coli by integrant expression of crtEBI gene and deletion of aceE and gdhA  

作　　者：Xiaoqing Hu Mao Cui Xiaoyuan Wang 

机构地区：[1]State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi 214122,China [2]International Joint Laboratory on Food Safety,Jiangnan University,Wuxi 214122,China

出　　处：《Systems Microbiology and Biomanufacturing》2023年第4期739-749,共11页系统微生物学与生物制造（英文）

基　　金：supported by the National Key research and Development Program of China(2018YFA0900302);the National Science Foundation of China(31970045);the National First-class Discipline Program of Light Industry Technology and Engineering(LITE2018-12);the Program of Introducing Talents of Discipline to Universities(111-2-06);Top-notch Academic Programs Project of Jiangsu Higher Education Institutions.

摘　　要：As a bright red carotenoid pigment found in tomatoes and other red fruits,lycopene was efficiently produced by Escherichia coli transformed with the genes involving in lycopene biosynthesis.Our previous work showed that E.coliΔwaaC andΔwaaF with higher permeability of outer membrane were better chassis for lycopene biosynthesis.However,further work needed to improve lycopene synthesis in the aforementioned strains.In the current study,the exogenous crtEBI genes from C.glutamicum 14067 were first integrated into the chromosome ofΔwaaC andΔwaaF.Compared toΔwaaC/pWSK29-crtEBI andΔwaaF/pWSK29-crtEBI(4.19 and 4.20 mg/g lycopene respectively),ΔwaaC lacZ::crtEBI(CWC01)andΔwaaF lacZ::crtEBI(CWF01)produced higher lycopene levels at 16.14 mg/g and 15.81 mg/g,respectively.Later,the individual and combinational deletion of aceE and gdhA was conducted in CWC01 and CWF01,respectively.The double knock-out strains CWC04 and CWF04 showed the improved yield at 19.65 mg/g and 22.24 mg/g,respectively.Finally,the four genes involving in MEP pathway(dxs,dxr,ispA and idi)were further overexpressed.The highest lycopene yield was achieved at 25.82 mg/g by CWF04/pACYC184-dxs^(E289G)-dxr^(K37N,K217N)-ispA-idi.The current study showed that E.coliΔwaaC andΔwaaF were optimal chassis for ambitious metabolic modification to produce lycopene.

关 键 词：LYCOPENE Escherichia coli waaC waaF 

分 类 号：Q78[生物学—分子生物学]