不同浓度镉诱导鸡心脏炎性损伤的机理研究  

Research of the mechanism on inflammatory injury of chickenheart induced by different concentrations of cadmium

作　　者：郭恺 李雪楠 李金龙[1] GUO Kai;LI Xuenan;LI Jinlong(College of Veterinary Medicine,Northeast Agricultural University,Harbin,Heilongjiang Province 150030,China;Chifeng Agricultural and Animal HusbandryTechnology Promotion Center,Chifeng,Inner Mongolia 024000,China)

机构地区：[1]东北农业大学,黑龙江哈尔滨150030 [2]赤峰市农牧技术推广中心,内蒙古赤峰024000

出　　处：《中国饲料》2024年第17期61-66,共6页China Feed

基　　金：国家自然科学基金项目(31572586)。

摘　　要：为研究不同浓度镉对鸡慢性中毒的心脏损伤及炎症的作用,本试验通过建立海兰白鸡亚慢性镉中毒模型,在饲料添加氯化镉诱导鸡的心脏炎性损伤。研究分为3组:对照组(Con)、35、70 mg/kg组,连续饲喂90 d。饲喂结束后,取心脏,检测心脏病理组织学、心脏功能指标、血清炎症指标、一氧化氮合酶(NOS)活性等。结果显示:不同浓度镉导致鸡心脏形态和病理组织结构改变,心脏炎性浸润明显增加,心脏功能指标异常。镉中毒导致心脏NOS酶活性增加,总一氧化氮合酶(T-NOS)和诱导型一氧化氮合酶(iNOS)活性增加(P<0.01和P<0.001),结构型一氧化氮合酶(cNOS)活性不受影响。此外,镉中毒导致心脏组织炎性细胞因子白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、白介素-8(IL-8)、核因子激活的B细胞的κ-轻链增强(NF-κB)水平明显增加(P<0.05、P<0.01、P<0.001),同时,血清IL-6、TNF-α、白介素-1β(IL-1β)含量水平升高(P<0.05、P<0.01)。综上,镉中毒能够激活炎性因子表达及分泌,导致鸡心脏功能障碍及炎性损伤。To investigate the effects of different concentrations of cadmium(Cd)on heart damage and inflammation in chickens with chronic poisoning,a subchronic Cd poisoning model was established in Hailan-white chickens.Cd was added to feed to induce heart inflammatory damage in chickens.The study was divided into three groups:control group,35 mg/kg group and 70 mg/kg group,which were fed continuously for 90 days.After feeding,the heart was taken and cardiac histopathology,cardiac function indicators,serum inflammation indicators,and NOS enzyme activity were tested.The results showed that different concentrations of Cd led to changes in the morphology and pathological tissue structure of the chicken heart.Significantly increased of inflammatory infiltration of the heart and abnormal cardiac function indicators were obviously.Moreover,Cd caused an increase in cardiac Nitric oxide synthases(NOS)enzyme activity,total NOS(T-NOS)and inducible NOS(iNOS)activities(P<0.01,P<0.001),while constitutive NOS(cNOS)activity was not affected by Cd.In addition,Cd triggered significantly increased inflammatory cytokines of interlekin(IL)-6 and tumor necrosis factor alpha(TNF-α),IL-8,nuclear factor-κB(NF-κB)in mRNA levels in heart tissue(P<0.05,P<0.01,P<0.001),with a similar increase of content of serum IL-6,TNF-αand IL-1β(P<0.05,P<0.01).In conclusion that Cd could activate the expression and secretion of inflammatory factors and led to cardiac dysfunction and inflammatory damage in chickens.

关 键 词：镉 心脏 鸡 炎症 

分 类 号：S858.31[农业科学—临床兽医学]