饲粮蛋白质水平和枯草芽孢杆菌制剂对大骨鸡种母鸡生产性能、蛋品质、养分表观代谢率及血清抗氧化和免疫指标的影响  

Effects of dietary crude protein levels and Bacillus subtilis preparationon performance,egg quality,nutrient apparent metabolic rate,serum antioxidant and immune indices of Dagu breeding hens

作　　者：靳昌旻 邵彩梅 于滢 任双 刘凤侠 管秀界 史东辉 JIN Changmin;SHAO Caimei;YU Ying;REN Shuang;LIU Fengxia;GUAN Xiujie;SHI Donghui(College of Animal Science and Veterinary Medicine,Jinzhou Medical University,Jinzhou,Liaoning Province 121001,China;Wellhope Foods Co,Ltd.,Shenyang,Liaoning Province 110121,China;Agricultural Comprehensive Law Enforcement Team,Huludao,Liaoning Province 125001,China)

机构地区：[1]锦州医科大学畜牧兽医学院,辽宁锦州121001 [2]禾丰食品股份有限公司,辽宁沈阳110121 [3]葫芦岛市农业综合行政执法队,辽宁葫芦岛125001

出　　处：《中国饲料》2024年第17期75-82,共8页China Feed

基　　金：辽宁省科学技术厅首批揭榜挂帅科技攻关项目(2021JH1/10400033)。

摘　　要：为探讨饲粮蛋白质水平和枯草芽孢杆菌制剂对大骨鸡生产性能、蛋品质、养分表观代谢率及血清生化指标的影响,试验选取38周大骨鸡576只,采用3×2双因子完全随机试验设计:PL0(蛋白质15.33%+枯草芽孢杆菌0 mg/kg);PM0(蛋白质15.90%+枯草芽孢杆菌0 mg/kg);PH0(蛋白质16.50%+枯草芽孢杆菌0 mg/kg);PL1(蛋白质15.33%+枯草芽孢杆菌200 mg/kg);PM1(蛋白质15.90%+枯草芽孢杆菌200 mg/kg);PH1(蛋白质16.50%+枯草芽孢杆菌200 mg/kg)。结果表明:与饲粮蛋白质15.33%组相比,15.90%和16.50%组大骨鸡的产蛋率提高了2.64%和3.28%;平均蛋重、平均总蛋重、蛋白高度、表观代谢能、干物质和粗蛋白质表观代谢率显著提高(P<0.05);料蛋比降低6.27%和7.12%(P<0.05);枯草芽孢杆菌制剂组大骨鸡蛋壳强度、蛋壳厚度、哈氏单位提高4.93%、5.41%和3.00%(P<0.05);表观代谢能,干物质、粗蛋白质、钙和磷的表观代谢率,血清钙、磷,谷胱甘肽过氧化物酶活性及IgG和IgA水平亦显著提高(P<0.05),破软蛋率、丙二醛含量分别降低1.16%和14.42%(P<0.05);16.50%蛋白质饲粮组大骨鸡血清谷胱甘肽过氧化物酶活性比15.33%蛋白水平组升高6.94%(P<0.05),饲粮蛋白质水平和枯草芽孢杆菌制剂均无互作效应(P>0.05)。综合各项指标得出,蛋白质水平为15.90%较适宜大骨鸡种母鸡的生产需要,枯草芽孢杆菌制剂可以提高大骨鸡鸡蛋品质和养分代谢率。This experiment was conducted to study the effects of the level protein and Bacillus subtilis preparation in diet on performance,egg quality,nutrient apparent metabolic rate and serum indices of Dagu breeding hens.A 3×2 two factors completely randomized experimental design was adopted with total of 57638-week-old birds:PL0(crude protein 15.33%+Bacillus subtilis 0 mg/kg);PM0(crude protein 15.90%+Bacillus subtilis 0 mg/kg);PH0(crude protein 16.50%+Bacillus subtilis 0 mg/kg);PL1(crude protein 15.33%+Bacillus subtilis 200 mg/kg);PM1(crude protein 15.90%+Bacillus subtilis 200 mg/kg);PH1(crude protein 16.50%+Bacillus subtilis 200 mg/kg).The experiment lasted for 33 days.The results showed that:the laying rate of Dagu breeding hens fed the dietary protein levels with 15.90%and 16.50%were higher by 2.64%and 3.28%compared to those fed dietary protein levels with 15.33%(P<0.05),also average egg weight,average total egg weight,albumen height,apparent metabolic energy,the apparent metabolic rates of dry matter and crude protein was significantly improved(P<0.05).Furthermore,the feed to egg ratio significantly decreased by 6.27%and 7.12%(P<0.05).The eggshell strength,eggshell thickness and Haugh units of the birds in the diet supplemented Bacillus subtilis preparation(BSP)groups improved 4.93%,5.41%and 3.00%(P<0.05),respectively.And also the apparent metabolic energy,the apparent metabolic rate of dry matter,crude protein,calcium and phosphorus were significantly improved(P<0.05).At the same time,the capacity of serum glutathione peroxidase activity,the level of serum calcium and serum phosphorus,the level of serum IgG and IgA of birds fed diet supplemented Bacillus subtilis preparation significantly improved(P<0.05);Simultaneously,the broken soft egg rate and the level of serum malondialdehyde significantly decreased by 1.16%and 14.42%(P<0.05).Dietary 16.50%protein level significantly increased serum capacity of glutathione peroxidase,but had no significant effect on other serum indexes(P>0.05).According to the comprehensiv

关 键 词：大骨鸡种鸡 蛋白质 枯草芽孢杆菌制剂 生产性能 养分表观代谢率 

分 类 号：S816.7[农业科学—饲料科学]