连翘叶乙醇提取物对APAP诱导肝损伤小鼠氧化应激及炎症因子的影响  

Effects of FSLE on oxidative stress and inflammatory factors in APAP-induced liver injury mice

作　　者：王中一 张治杰 姚亚乐 陈国雯 马小燕 安志霞 范碧玥 邱山桐 王萌[1] WANG Zhongyi;ZHANG Zhijie;YAO Yale;CHEN Guowen;MA Xiaoyan;AN Zhixia;FAN Biyue;QIU Shantong;WANG Meng(College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070

出　　处：《甘肃农业大学学报》2024年第4期1-8,共8页Journal of Gansu Agricultural University

基　　金：国家自然科学基金项目(32160850);甘肃省自然科学基金项目(22JR5RA868);甘肃农业大学青年导师扶持基金项目(GAU-QDFC-2021-05)。

摘　　要：【目的】研究连翘叶乙醇提取物(ethanolic extract of Forsythia suspense leaves,FSLE)对对乙酰氨基酚(acetaminophen,APAP)诱导的肝损伤作用及其机制。【方法】随机将48只昆明小鼠分为空白对照组(NC组)、APAP肝损伤模型组(LD组)、连翘叶乙醇提取物高剂量对照组、高、中、低剂量组(FSLE组、HFSLE+LD组、MFSLE+LD组、LFSLE+LD组),每组8只。建立肝损伤模型并给药,检测各组小鼠谷丙转氨酶(ALT)、谷草转氨酶(AST)、超氧化物歧化酶(SOD)活性与谷胱甘肽(GSH)、丙二醛(MDA)和过氧化氢(H2O2)含量,苏木精伊红(HE)染色观察肝组织病理情况,检测TRAF2、NF-κB p65 mRNA、蛋白的表达水平和肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)和白细胞介素6(IL-6)的水平。【结果】FSLE可降低肝损伤小鼠ALT、AST活性和MDA、H2O2含量(P<0.05),提高SOD活性和GSH含量(P<0.05),改善肝组织病理情况,降低TRAF2和NF-κB p65 mRNA、蛋白表达量和TNF-α、IL-1β、IL-6的水平(P<0.05)。【结论】FSLE通过改善氧化应激、调节TNF/NF-κB p65信号通路和抑制炎症反应发挥抗APAP肝损伤的作用。【Objective】This study aimed to investigate the impact of the ethanolic extract of Forsythia leaves(FSLE)on acetaminophen(APAP)-induced liver injury and its underlying mechanism.【Method】Forty-eight Kunming mice were randomly assigned to six groups:the blank control group(NC),APAP liver injury model group(LD),FSLE control group(FSLE),and high,medium,and low doses of FSLE treatment groups(HFSLE+LD,MFSLE+LD,and LFSLE+LD),with eight mice in each group.The liver injury model was established,and drug administration took place.The levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),superoxide dismutase(SOD)activity,glutathione(GSH),malondialdehyde(MDA),and hydrogen peroxide(H2O2)were assessed in each group of mice.Liver histopathological changes were observed through hematoxylin-eosin staining(HE).TRAF2,NF-κB p65 mRNA,and protein expression levels were measured,along with TNF-α,IL-1β,and IL-6 levels.【Result】FSLE reduced ALT and AST activity,MDA,and H2O2 content,while increasing SOD activity and GSH content.It also ameliorated liver histopathology,decreased TRAF2 and NF-κB p65 mRNA and protein expression,and lowered TNF-α,IL-1β,and IL-6 levels in mice with liver injury.【Conclusion】In conclusion,FSLE demonstrated hepatoprotective effects against APAP-induced liver injury by mitigating oxidative stress,modulating the TNF/NF-κB p65 signaling pathway,and suppressing the inflammatory response.

关 键 词：连翘叶乙醇提取物 肝损伤 小鼠 氧化应激 炎症因子 

分 类 号：S683.24[农业科学—观赏园艺]