水草组培污染防治实验教学改革  

Water Grass Tissue Culture Pollution Prevention and Control in experimental teaching reform

作　　者：姜炳阳 曹广龙 李伟杰 赵震扬 郭雅宁 王梅 梁梦婷 郭智勇 管连庆 马雯雯 刘明慧 孙验玲 JIANG Bingyang;CAO Guanglong;LI Weijie;ZHAO Zhenyang;GUO Yaning;WANG Mei;LIANG Mengting;GUO Zhiyong;GUAN Lianqing;MA Wenwen;LIU Minghui;SUN Yanling(School of Marine Science and Engineering,Qingdao Agricultural University,Qingdao 266237,China)

机构地区：[1]青岛农业大学海洋科学与工程学院,山东青岛266237

出　　处：《安徽农学通报》2024年第17期105-108,共4页Anhui Agricultural Science Bulletin

基　　金：国家自然科学基金青年基金(31902408);青岛农业大学实验技术研究课题(SYJS202326);青岛农业大学课程思政教育专项(XJY2022141);青岛农业大学博士基金(1120035);山东省自然科学基金面上项目(ZR2023MC154)。

摘　　要：为探究水草组织培养污染的污染源及药物防治措施,本研究对水草组培污染防治实验训练进行教学设计,采用水草组织细胞培养课程与水草组培污染防治实验相结合的教学模式,对教学目标与教学内容进行探讨,并分析实验内容及流程。以典型的水草红丝青叶(Hygrophila polysperma)为对象进行组织培养,通过从污染菌中进行细菌和真菌的分离和纯化,采用16S rDNA和ITS基因测序,并结合表型分析进行种类鉴定,明晰了污染菌的种类。进一步对污染菌开展防治药物筛选,通过药敏实验获得了有效的药物组合,加入培养基进行组培培养基优化,取得了一定的抗菌防治效果。该教学模式有利于学生熟练掌握水草组培污染防治技术原理、方案及应用等,为水草扩繁生产和相关科学研究培养专业人才。To explore the pollution sources and drug prevention measures of water plant tissue culture pollution,teaching was designed for the experimental training of water grass tissue culture pollution prevention and control,a teaching model was adopted that combined water grass tissue cell culture courses with water grass tissue culture pollution prevention experiments,the teaching objectives and content were explored,and the experimental content and process were analyzed.Tissue culture on typical common water plant Hygrophila polysperma were conducted,by isolating and purifying the contaminating bacteria,16S rDNA and ITS gene sequencing were used in combination with phenotype analysis for species identification,clarifying the source of the contaminating bacteria.Further screening of prevention and control drugs for cotaminants was carried out,and effective drug combinations were obtained through drug sensitivity experiments.The addition of culture medium for tissue culture medium optimization achieved certain antibacterial and control effects.This course showed that this teaching model was beneficial for students to proficiently master the principles,schemes,and applications of water grass tissue culture pollution prevention and control technology,to cultivate professional talents for the expansion and production of aquatic plants and related scientific research.

关 键 词：组织培养 红丝青叶 细菌 真菌 污染防治 

分 类 号：S451[农业科学—植物保护] G642.0[文化科学—高等教育学]