棉花抗黄萎病相关基因GhERF020功能的初步分析  

Preliminary Function Analysis of GhERF020 Gene in Response to Verticillium Wilt in Cotton

作　　者：翁慧婷 刘海洋[3] 郭惠明[4] 程红梅[4] 李君 张超 苏晓峰[4] WENG Huiting;LIU Haiyang;GUO Huiming;CHENG Hongmei;LI Jun;ZHANG Chao;SU Xiaofeng(College of Life Science,Hebei Agricultural University,Hebei Baoding 071001,China;Hebei Bioinformatic Utilization and Technological Innovation Center for Agricultural Microbes,Hebei Baoding 071001,China;Institute of Plant Protection,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China;National Key Laboratory of Agriculture Microbiology,Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]河北农业大学生命科学学院,河北保定071001 [2]河北省农业微生物生物信息利用技术创新中心,河北保定071001 [3]新疆农业科学院植物保护研究所,乌鲁木齐830091 [4]中国农业科学院生物技术研究所,农业微生物资源发掘与利用全国重点实验室,北京100081

出　　处：《中国农业科技导报》2024年第9期112-121,共10页Journal of Agricultural Science and Technology

基　　金：河北省重点研发计划项目(22322912D);国家自然科学基金项目(32160624)。

摘　　要：黄萎病(Verticillium wilt)是影响我国棉花产量和品质的重要病害,其病原菌是大丽轮枝菌(Verticillium dahliae)。AP2/ERF类转录因子在植物生长发育和胁迫适应性响应中的作用日益突显。为挖掘棉花抗黄萎病相关基因并探究其生物学功能,利用拟南芥抗大丽轮枝菌相关基因AT1G22810.1,同源比对获得棉花抗黄萎病候选基因GhERF020(XM_016842745.1)。生物信息学分析结果表明,GhERF020开放阅读框的长度为441 bp,编码147个氨基酸;其蛋白含有1个保守的AP2结构域,相对分子质量为16.16 kD,无跨膜结构,属于亲水蛋白;预测其启动子区域含有水杨酸、茉莉酸甲酯和脱落酸等响应元件。基因表达模式分析发现,GhERF020受大丽轮枝菌侵染诱导表达。亚细胞定位分析表明,GhERF020蛋白定位于细胞核中。利用病毒诱导的基因沉默(virus-induced gene silencing,VIGS)技术下调GhERF020表达后,棉花植株对大丽轮枝菌的敏感性显著增强,病情指数和病原菌生物量明显升高。由此表明,GhERF020是棉花抗黄萎病的正向调控因子。Verticillium wilt(VW)is a disruptive symptom caused by Verticillium dahliae,which affects fiber yield and quality of cotton in China.AP2/ERF transcription factors play prominent role in plant growth,development and stress response.In order to excavate the genes related to resistance to VW in cotton and explore their biological functions,the candidate gene GhERF020(XM_016842745.1)was obtained by homologous alignment of AT1G22810.1 related gene of Arabidopsis thaliana against V.dahliae.Bioinformatic analysis showed that the open reading frame length of GhERF020 was 441 bp,encoded 147 amino acids.GhERF020 contained a conserved AP2 domain,the relative molecular weight was 16.16 kD without transmembrane structure,belonging to the hydrophilic protein.It was predicted that the promoter region contained response elements of salicylic acid(SA),methyl jasmonate(MEJA),abscisic acid(ABA)and other response elements.The expression of GhERF020 was induced by V.dahliae infection.Subcellular localization analysis showed that GhERF020 protein was localized in the nucleus.As the expression of GhERF020 was downregulated by virus-induced gene silencing(VIGS)technique,the susceptibility of cotton plants to V.dahliae was significantly enhanced,and the disease index and pathogen biomass were distinctly increased.Above results indicated that GhERF020 was a positive regulator in resistance against V.dahliae in cotton.

关 键 词：棉花黄萎病 大丽轮枝菌 转录因子 GhERF020 VIGS 

分 类 号：S562[农业科学—作物学] Q78[生物学—分子生物学]