吡咯喹啉醌对运动性疲劳小鼠血清代谢组学的影响  

Effect of Pyrroloquinoline Quinone on Serum Metabolomics of Mice with Exercise-Induced Fatigue

作　　者：徐鑫镁[1] 林景鸿 邹文杰 汪亮 潘鑫杰 欧明珊 刘丽霞[1,2,3] XU Xin-mei;LIN Jing-hong;ZOU Wen-jie;WANG Liang;PAN Xin-jie;OU Ming-shan;LIU Li-xia(School of Physical Education and Sport Science,Fujian Normal University,Fuzhou 350000,China;Shanghai University of Sport,Shanghai 200000,China;Biomedical Research Center of South China,Fujian Normal University,Fuzhou 350315,China)

机构地区：[1]福建师范大学体育科学学院,福建福州350000 [2]上海体育大学,上海200000 [3]福建师范大学南方生物医学研究中心,福建福州350315

出　　处：《福建体育科技》2024年第4期23-32,50,共11页Fujian Sports Science and Technology

基　　金：福建省自然科学基金项目(2021J01174);国家自然科学基金项目(22377012)。

摘　　要：目的:探讨吡咯喹啉醌(PQQ)对运动性疲劳小鼠血清代谢组学的影响,寻找关键标志物,揭示PQQ在缓解运动疲劳中的代谢途径及其潜在的调节机制。方法:采用反复力竭游泳构建运动性疲劳的小鼠模型,进行为期两周的PQQ补充干预,剂量为每日10 mg/kg,利用色谱与质谱联合技术对小鼠血清中的代谢产物进行检测,并对筛选出的关键代谢物及其相关的代谢路径进行详细分析。结果:(1)反复力竭后,运动性疲劳小鼠与正常安静小鼠相比,血清中有32种差异代谢物;(2)反复力竭后,补充PQQ小鼠,与没有补充PQQ的小鼠相比,血清中有10种潜在差异代谢物;(3)进一步的组间比较揭示了两组小鼠中共有的4种显著差异的代谢物,包含N-乙酰-L-苯丙氨酸、柠檬酸、木糖醇及L-脯氨酸;(4)代谢通路分析显示,主要的差异代谢路径包括由柠檬酸参与的三羧酸循环中的乙醛酸循环支路和木糖醇参与的糖醛酸途径。结论:PQQ在维持运动性疲劳小鼠的正常血清代谢水平上发挥着关键作用,其所具有的作用机制或许与调控柠檬酸、木糖醇、L-脯氨酸以及N-乙酰-L-苯丙氨酸等关键代谢物及其所属的代谢通路存在紧密关联。Objective:To explore the effect of pyrroloquinoline quinone(PQQ)on serum metabolomics in mice with exercise-induced fatigue,seek out key markers,and uncover the metabolic pathway as well as the potential regulatory mechanism of PQQ in alleviating exercise-induced fatigue.Methods:the mouse model of exercise-induced fatigue was established by repeated exhaustive swimming,and PQQ was supplemented for two weeks at a dose of 10 mg/kg per day.The metabolites in the serum of mice were detected by chromatography and mass spectrometry,and the key metabolites screened and their related metabolic pathways were analyzed in detail.Results:(1)after repeated exhaustion,there were 32 different metabolites in the serum of exercise-induced fatigue mice compared with normal quiet mice;(2)After repeated exhaustion,PQQ supplemented mice had 10 potential differential metabolites in serum compared with mice without PQQ supplementation;(3)Further comparison between groups revealed four significantly different metabolites in the two groups of mice,namely citric acid,xylitol,L-proline and n-acetyl-l-phenylalanine;(4)The main differential metabolic pathways included the glyoxylate cycle branch in the tricarboxylic acid cycle involving citric acid and the glyoxylate pathway involving xylitol.Conclusion:PQQ exerts a significant role in sustaining the normal serum metabolic level of mice with exercise-induced fatigue.,and its mechanism may be closely related to the regulation of key metabolites such as citric acid,xylitol,L-proline and n-acetyl-l-phenylalanine and their metabolic pathways.

关 键 词：运动性疲劳 血清代谢组学 吡咯喹啉醌 

分 类 号：G804.7[文化科学—运动人体科学]