喜马拉雅旱獭血清中鼠疫噬菌体特异性抗体检测  

Detection of specific antibodies to plague phage in serum of Marmota Himalayana

作　　者：杨建国[1] 游培松 张晓璐 李光辉 李翔[1] 李胜[1] 冯建萍[1] 金泳[1] 赵海红[1] 王永顺[1] 谷昕玥 李存香[1] YANG Jianguo;YOU Peisong;ZHANG Xiaolu;LI Guanghui;LI Xiang;LI Sheng;FENG Jianping;JIN Yong;ZHAO Haihong;WANG Yongshun;GU Xinyue;LI Cunxiang(NHC Key Laboratory of Plague Control and Research(Qinghai Institute for Endemic Disease Prevention and Control),Xining,Qinghai 810021,China)

机构地区：[1]国家卫生健康委鼠疫防治研究重点实验室(青海省地方病预防控制所),青海西宁810021

出　　处：《医学动物防制》2024年第8期770-773,共4页Journal of Medical Pest Control

基　　金：青海省科技厅基础研究计划项目(2022-ZJ-750);青海省医药卫生科技项目重点课题(2022-wjzd-06);第二次青藏高原综合科学考察研究资助(2019QZKK0607);青海省科技计划项目——青海省突发重大传染病疫情防控公共卫生服务能力提升(2023-QY-202)。

摘　　要：目的利用微量板法间接检测青海省兴海县喜马拉雅旱獭血清中特异性鼠疫噬菌体抗体,为该地区鼠疫流行态势分析、噬菌体与哺乳动物机体免疫系统的相互作用提供理论依据。方法采用微量板法经间接血细胞凝集试验,检测497份采集于2020—2021年青海省兴海县喜马拉雅旱獭血清中的鼠疫耶尔森菌荚膜蛋白FraI抗体,再以1株温和性鼠疫噬菌体和1株裂解性鼠疫噬菌体为抗原,采用微量板法和双层琼脂平板法间接检测喜马拉雅旱獭血清中的鼠疫噬菌体抗体。结果采用微量板法经间接血细胞凝集试验检测497份喜马拉雅旱獭血清中鼠疫耶尔森菌荚膜蛋白FraI抗体,均为阴性;其次497份血清分别与2株不同类型鼠疫噬菌体进行中和试验,基于点滴法在上述血清中均未检测到鼠疫噬菌体免疫抗体,间接提示兴海县鼠疫耶尔森菌的数量可能与特定噬菌体的出现相关。结论2010—2019年兴海县鼠疫疫源地未发现动物间鼠疫流行,喜马拉雅旱獭血清中未检出特异性鼠疫噬菌体抗体可能与当地的鼠疫流行形式和强度有关。Objective To detect specific antibodies to plague phage in the serum of Marmota Himalayana in Xinghai County,Qinghai Province indirectly by microassay,to provide theoretical basis for the analysis of the epidemiological situation of plague in this region and the interaction between phage and mammalian immune system.Methods The microplate method was used to detect the antibody to Yersin pestis capsular protein FraI in 497 sera of Marmota Himalayana collected in 2020 to 2021 in Xinghai County,Qinghai Province by indirect hemagglutination test.The microplate method and double-layer agar plate method were used to indirectly detect plague phage antibodies in sera of Marmota Himalayana,using one mild strain of plague phage and one lysogenic strain of plague phage as antigens.Results Antibody to Yersin pestis capsular protein FraI were detected in 497 sera of Marmota Himalayana by indirect hemagglutination test using the microplate method,with all negative.Secondly,497 sera were neutralized with 2 strains of different types of plague phage,and no plague phage antibody was detected in the above sera based on dot-drop method,which indirectly suggested the number of Yersinia pestis in Xinghai County may be associated with the presence of specific phages.Conclusion No animal plague epidemic is detected in the plague focus in Xinghai County from 2010 to 2019.The absence of specific antibody to plague phage in the sera of Marmota Himalayana might be related to the endemic form and intensity of the plague.

关 键 词：噬菌体 喜马拉雅旱獭 血清 免疫抗体 流行病学 鼠疫 

分 类 号：R378.61[医药卫生—病原生物学]