川芎嗪对铝致认知功能障碍大鼠学习记忆功能的影响及机制  

Effect of ligustrazine on learning and memory function of rats with aluminum-induced cognitive impairment and its mechanism

作　　者：李晨羽 贾芸菁 张邱晟熙 黄作著 刘洋 凌雁武 LI Chenyu;JIA Yunjing;ZHANG Qiushengxi;HUANG Zuozhu;LIU Yang;LING Yanwu(School of Basic Medicine,Youjiang Medical University for Nationalities,Baise 533000,Guangxi Zhuang Autonomous Region,China)

机构地区：[1]右江民族医学院基础医学院,广西百色533000

出　　处：《新乡医学院学报》2024年第9期801-808,共8页Journal of Xinxiang Medical University

基　　金：国家自然科学基金资助项目(编号:31560294);广西高校中青年教师科研基础能力提升项目(编号:RZ2300000977);广西高校中青年教师科研基础能力提升项目(编号:2023KY0556)。

摘　　要：目的探讨川芎嗪对铝致认知功能障碍大鼠学习记忆功能的影响及机制。方法按随机数字表法将60只Wistar雄性大鼠分为空白对照组、模型组、低剂量川芎嗪组、高剂量川芎嗪组和吡拉西坦组,每组12只。空白对照组大鼠不做任何处理;模型组、低剂量川芎嗪组、高剂量川芎嗪组和吡拉西坦组大鼠首先使用100 mg·kg^(-1)的三氯化铝溶液每日灌胃制备铝中毒模型;造模成功后,吡拉西坦组大鼠用吡拉西坦按400 mg·kg^(-1)剂量灌胃,低剂量川芎嗪组和高剂量川芎嗪组大鼠分别给予100、200 mg·kg^(-1)川芎嗪灌胃,空白对照组和模型组大鼠每日灌胃同体积的生理盐水;5组大鼠均每日灌胃1次,连续灌胃30 d。干预30 d后,应用Morris水迷宫实验评估各组大鼠的学习、记忆功能;待完成水迷宫实验后,应用200 g·L^(-1)水合氯醛对大鼠实施麻醉后,迅速断头取脑组织,应用免疫组织化学染色法观察5组大鼠海马CA1区中电压依赖性钙通道α1E亚基(CACNA1E)、钙调蛋白(CALM)、脑源性神经营养因子(BDNF)表达情况,Western blot法检测5组大鼠海马CA1区CACNA1E、CALM和BDNF蛋白相对表达量,实时荧光定量聚合酶链反应检测5组大鼠海马CA1区CACNA1E、CALM和BDNF mRNA相对表达量。结果Morris水迷宫实验第1天,模型组、吡拉西坦组、低剂量川芎嗪组和高剂量川芎嗪组大鼠的潜伏期显著高于空白对照组(P<0.05);吡拉西坦组、低剂量川芎嗪组、高剂量川芎嗪组、模型组大鼠的潜伏期比较差异无统计学意义(P>0.05)。Morris水迷宫实验第3天,模型组、吡拉西坦组、低剂量川芎嗪组和高剂量川芎嗪组大鼠的潜伏期显著高于空白对照组,吡拉西坦组和高剂量川芎嗪组大鼠的潜伏期显著低于模型组和低剂量川芎嗪组(P<0.05);低剂量川芎嗪组与模型组大鼠的潜伏期比较差异无统计学意义(P>0.05)。Morris水迷宫实验第5天,模型组、吡拉西坦组、低剂量Objective To explore the effect of ligustrazine on the learning and memory function of rats with aluminum-induced cognitive impairment and its mechanism.Methods Sixty male Wistar rats were divided into a blank control group,a model group,a low-dose ligustrazine group,a high-dose ligustrazine group,and a piracetam group using a random number table method,with 12 rats in each group.The rats in the blank control group were not subjected to any treatment;the rats in the model group,low-dose ligustrazine group,high-dose ligustrazine group,and piracetam group were first prepared with aluminum toxicity models by daily gavage of 100 mg·kg^(-1) AlCl3 solution.After successful modeling,the rats in the piracetam group were intragastrically administered with piracetam at a dose of 400 mg·kg^(-1),while rats in the low-dose and high-dose ligustrazine groups were intragastrically administered with 100 and 200 mg·kg^(-1) ligustrazine,respectively;the rats in the blank control group and the model group were intragastrically administered with the same volume of physiological saline.All rats in the five groups received intragastric administration once a day for 30 consecutive days.After 30 days of intervention,the Morris water maze test was used to evaluate the learning and memory function of rats in the five groups.After completing the water maze experiment,rats in the five groups were anesthetized with 200 g·L^(-1) chloral hydrate,and their brain tissues were quickly removed after decapitation.Immunohistochemical staining was used to observe the expression of calcium voltage-gated channel subunit alpha 1E(CACNA1E),calmodulin(CALM),and brain-derived neurotrophic factor(BDNF)in the hippocampal CA1 region of rats in the five groups;Western blot was used to detect the relative expression levels of CACNA1E,CALM,and BDNF proteins in the hippocampal CA1 region of rats in the five groups;and real-time fluorescence quantitative polymerase chain reaction was used to detect the relative expression levels of CACNA1E,CALM,and BDNF mRNA in

关 键 词：铝中毒 认知功能障碍 学习记忆功能 川芎嗪 海马 

分 类 号：R285.5[医药卫生—中药学]