槲皮素对生长期小鼠峰值骨量和脏器病理学的影响  

Effects of quercetin on peak bone mass and organ pathology in growing mice

作　　者：王龙飞 高玉海 杨世超 杨玉田 魏振龙 陈克明 WANG Longfei;GAO Yuhai;YANG Shichao;YANG Yutian;WEI Zhenlong;CHEN Keming(Basic Medical Laboratory,No.940 Hospital of Joint Logistics Support Force of Chinese PLA,Lanzhou 730050,China;College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070,China;Gansu Key Laboratory of Stem Cells and Genetic Drug,Lanzhou 730050,China)

机构地区：[1]中国人民解放军联勤保障部队第九四〇医院基础医学实验室,甘肃兰州730050 [2]甘肃农业大学生命科学技术学院,甘肃兰州730070 [3]甘肃省干细胞与基因药物重点实验室,甘肃兰州730050

出　　处：《西部医学》2024年第9期1270-1276,共7页Medical Journal of West China

基　　金：国家自然科学基金面上项目(81770879);甘肃省科技计划资助项目(22JR11RA013);联勤保障部队第九四〇医院实验室培育项目(2021yxky081);联勤保障部队第九四〇医院青年培育项目(2021yxky053)。

摘　　要：目的探究槲皮素对生长期小鼠峰值骨量和脏器病理学的影响。方法将40只SPF级C57雌性小鼠随机分为对照组(Control)、槲皮素低剂量组(Q-20)、槲皮素中剂量组(Q-40)和槲皮素高剂量组(Q-80)4组,每组各4只。Q-20组、Q-40组和Q-80组分别每天灌胃20、40和80 mg/kg槲皮素,Control组给予等体积蒸馏水。给药6周后取材,分别观察脏器系数、主要脏器病理学切片、骨组织病理学切片、分析Micro CT和蛋白质免疫印迹实验。结果槲皮素组主要的脏器系数与Control组相比,差异均无统计学意义(P>0.05);脏器病理学切片未发现明显的病变和毒副作用;骨组织脱钙切片Q-20组与Control组相比,脂肪细胞较少,骨小梁比较连贯和整齐;Q-20组、Q-40组和Q-80组的小鼠与Control组小鼠比较,数值在Tb.BMD、Tb.N、Tb.Th、Ct.BMD和Tb.BV/TV均有增加,但是Tb.Sp数值均有减少,差异均有统计学意义(P<0.05);Q-20组的OSX、COL-1、Runx-2与BMP-2的表达最为明显,差异有统计学意义(P<0.01)。结论槲皮素可能是通过增加骨密度、改善骨微结构、促进骨形成、从而提高峰值骨量,并且对脏器没有明显的副作用。Objective To investigate the effects of quercetin on peak bone mass and organ pathology in growing mice.Methods Forty SPF grade C57 female mice were randomly divided into 4 groups,with 10 mice in each group.They were divided into control group(Control),low dose quercetin group(Q-20),medium dose quercetin group(Q-40),and high dose quercetin group(Q-80).The Q-20,Q-40,and Q-80 groups were given 20,40,and 80 mg/kg quercetin by gavage every day,respectively.The control group was given equal volume distilled water.After 6 weeks of administration,samples were taken for observation of organ coefficients,pathological sections of main organs,pathological sections of bone tissue,analytical Micro CT,and protein immunoblotting experiments.Results There was no significant difference in the main organ coefficients between the quercetin group and the control group(P>0.05);No obvious pathological changes and toxic side effects were found in the pathological sections of the organs;Compared with control group,Q-20 group had fewer adipocytes and more consistent and neat bone trabeculae;Compared with control group mice,the values of Tb.BMD,Tb.N,Tb.Th,Ct.BMD,and Tb.BV/TV in Q-20,Q-40,and Q-80 groups increased,but the Tb.SP values decreased,with significant differences(P<0.05);The expression of OSX,COL-1,Runx-2,and BMP-2 in Q-20 group was the most significant,with a statistically significant difference(P<0.01).Conclusion Quercetin may increase peak bone mass by increasing bone density,improving bone microstructure,promoting bone formation,and has no significant side effects on organs.

关 键 词：槲皮素 峰值骨量 脏器病理学 骨组织脱钙 骨密度 

分 类 号：R681[医药卫生—骨科学]