miRNA-140-5P在甲状腺癌细胞TPC-1中的表达及对其增殖迁移侵袭能力的影响  

Expression of miRNA-140-5P in thyroid cancer cells TPC-1 and its effect on proliferation,migration and invasion

作　　者：罗义 李阳敏 鲜童丞 杨闵叶 王杰[1] 苏强 LUO Yi;LI Yangmin;XIAN Tongcheng;YANG Mingye;WANG Jie;SU Qiang(Beijing Anzhen Nanchong Hospital,Capital Medical University,Nanchong Central Hospital,Nanchong 637000,Sichuan,China;Department of Gynecology,Jialing Hospital,Nanchong Central Hospital,Nanchong 637000,Sichuan,China)

机构地区：[1]首都医科大学附属北京安贞医院南充医院·南充市中心医院乳腺甲状腺外科,四川南充637000 [2]南充市中心医院嘉陵院区妇科,四川南充637000

出　　处：《西部医学》2024年第9期1277-1281,共5页Medical Journal of West China

基　　金：四川省科技厅课题基金项目(2022NSFSC0711)。

摘　　要：目的探讨miR-140-5p在甲状腺癌发展中的作用及其分子生物调节机制,miRNA-140-5P对甲状腺癌细胞TPC-1侵袭、迁移的调控作用机制。方法收集2021年1月—2022年1月南充市中心医院手术切除的甲状腺恶性肿瘤36例和良性肿瘤32例的肿瘤标本。qt-PCR检测miRNA-140-5P在甲状腺恶性肿瘤和良性肿瘤组织中mRNA的表达水平,外购甲状腺癌细胞TPC-1,WB实验检测miRNA-140-5P在甲状腺恶性肿瘤和良性肿瘤细胞中的蛋白表达水平,评估miRNA-140-5P在两种组织中的表达差异;实验引入miRNA-140-5Pmimics转染细胞,Transwell小室实验、CCK-8对甲状腺肿瘤细胞迁移、侵袭、增殖能力进行检测;实验对甲状腺癌细胞TPC-1中NLRP3炎性小体进行检测,并对NLRP3表达进行干预,评估甲状腺肿瘤细胞中miR-140-5P与NLRP3的调控关系。结果miRNA-140-5P在甲状腺恶性肿瘤组织和细胞中呈低表达(P<0.05)。而miRNA-140-5P mimics可增强甲状腺癌细胞TPC-1的增殖、迁移和侵袭能力,miRNA-140-5P可减弱甲状腺癌细胞中炎性介质NLRP3的表达。结论miRNA-140-5P可降低甲状腺癌细胞TPC-1的增殖、迁移和侵袭能力;并在甲状腺恶性肿瘤细胞中与NLRP3炎性小体存在交互调控,其可能降低了对NLRP3的抑制作用,从而激发了NLRP3参与的活化调控而诱发了甲状腺癌的发生发展。Objective To explore the role of miR-140-5p in the development of thyroid cancer and its molecular biological regulation mechanism,the regulatory mechanism of miRNA-140-5P on the invasion and migration of thyroid cancer cells(TPC-1).Methods From January 2021 to January 2022,36 cases of thyroid malignant tumor and 32 cases of benign tumor were collected.The mRNA expression level of miRNA-140-5P in thyroid malignant and benign tumor tissues was detected by qt-PCR,and the protein expression level of miRNA-140-5P in thyroid malignant and benign tumor cells was detected by WB assay.The difference of miRNA-140-5P expression in the two tissues was evaluated;The experiment introduced miRNA-140-5Pmimics transfected cells,Transwell cell experiment,CCK-8 to detect the migration,invasion and proliferation of thyroid tumor cells.The experiment detected NLRP3 inflammatory corpuscles in thyroid cancer cells(TPC-1),and intervened in the expression of NLRP3 to evaluate the regulatory relationship between miR-140-5P and NLRP3 in thyroid cancer cells.Results miRNA-140-5P showed low expression in thyroid malignant tumor tissues and cells(P<0.05).However,miRNA-140-5Pmimics could enhance the proliferation,migration and invasion of thyroid cancer cell TPC-1,and reduce the expression of inflammatory mediator NLRP3 in thyroid cancer cells.Conclusion miRNA-140-5P can reduce the proliferation,migration and invasion of thyroid cancer cell TPC-1.There is interaction regulation with NLRP3 inflammatory body in thyroid malignant tumor cells,which may reduce the inhibition of NLRP3,thus stimulating the activation regulation of NLRP3 and inducing the occurrence and development of thyroid cancer.

关 键 词：miRNA-140-5P 甲状腺癌 增殖 迁移 侵袭 

分 类 号：R653.2[医药卫生—外科学]