锌指蛋白A20在低出生体重儿胎盘组织中的表达及其对滋养细胞炎症的影响  

Expression of A20 in placenta tissues of patients with low birth weight babies and its effect on trophoblastic inflammation

作　　者：高丽娜 董燕[1] 刘小晖[1] 刘小玲[1] 何晓春[1] 张玉芳[1] 张莉[1] 王秀娟 孙俊 高晶[1] 王玥元[2] GAO Lina;DONG Yan;LIU Xiaohui;LIU Xiaoling;HE Xiaochun;ZHANG Yufang;ZHANG Li;WANG Xiujuan;SUN Jun;GAO Jing;WANG Yueyuan(The Second Obstetrics Department,Gansu Provincial Maternity and Childcare Hospital,Lanzhou 730050,China;Department of Pathology,Gansu Provincial Maternity and Childcare Hospital,Lanzhou 730050,China)

机构地区：[1]甘肃省妇幼保健院产二科,甘肃兰州730050 [2]甘肃省妇幼保健院病理科,甘肃兰州730050

出　　处：《西部医学》2024年第9期1288-1294,共7页Medical Journal of West China

基　　金：甘肃省科技计划项目(22JR11RA176);2020年度“母婴临床研究专项基金”(2020BINCLC004)。

摘　　要：目的探讨胎盘组织锌指蛋白A20(A20)的表达水平与低出生体重儿(LBW)新生儿体重及胎盘体重的相关性及其在LBW发病机制中的作用。方法选取2021年9月—2022年5月在本院住院分娩的LBW孕妇30例为LBW组,同期健康妊娠分娩的孕妇30例为健康对照组(NP组);qRT-PCR和Western blot检测两组胎盘组织A20的表达,采用免疫组化检测胎盘组织中A20表达水平;然后分别将A20的表达水平与LBW组新生儿体重及胎盘体重做相关性分析。同时体外培养人源胎盘滋养细胞株(HTR-8/SVneo),分为对照组(NC组)和单纯LPS刺激组(100 ng/mL),转染A20基因腺病毒过表达后,Western blot检测各组胎盘滋养细胞A20蛋白、NF-κB结合蛋白p65、NF-κB亚单位磷酸化p65(p-p65)和TNF-α和IL-1β蛋白的表达,双抗体夹心酶联免疫吸附测定(ELISA)方法检测上清液TNF-α、IL-1β的表达。结果与NP组比较,LBW组年龄、孕周差异无统计学意义(P>0.05),而新生儿体重、胎盘重量、Apgar评分1 min,5 min差异均有统计学意义(P<0.05);A20在LWB组中明显低表达(P<0.01),免疫组化显示A20在LBW组胎盘组织中降低,且与新生儿体重及胎盘重量呈正相关(P<0.05);与NC组比较,单纯LPS刺激组细胞A20蛋白表达降低(P<0.05),而p-p65和TNF-α和IL-1β蛋白表达降低(P<0.05);转染A20基因腺病毒过表达后,A20表达增加(P<0.05),p-p65和TNF-α和IL-1β蛋白表达降低(P<0.05)。结论A20与LBW的发生发展密切相关,其过表达可有效抑制LPS诱导的滋养细胞炎症因子的产生,有望成为预测LWB的分子标记物和治疗靶点。Objective To investigate the zinc finger protein A20(also known as tumor necrosis factor-induced protein 3)in placenta and correlation between TNFAIP3 expression level and neonatal weight and placental weight in Low birth weight babies(LBW)and its role in the pathogenesis of LBW.Methods Thirty pregnant women with low birth weight babies(LBW)who delivered in Gansu Provincial Maternal and Child Health Hospital from September 2021 to May 2022 were selected.30 healthy pregnant women who delivered during the same period were selected as normal pregnancy(NP).qRT-PCR and Western blot were used to detect the expression of A20 in placental tissues of the two groups.The immunohistochemistry was used to detect the expression level of A20 in placental tissues.The correlation between the expression level of A20 and neonatal weight and placental weight in LBW group was analyzed.At the same time,human placental trophoblast cells(HTR-8/SVneo)were cultured in vitro and divided into control group(NC)and LPS group(100ng/mL).After overexpression of adenovirus A20 gene,the expressions of A20 protein,NF-κB binding protein p65,p-p65,TNF-αand IL-1βin placental trophoblast cells were detected by Western blot.The expressions of TNF-αand IL-1βin supernatant were detected by ELISA.Results Compared with the NP group,there were no significant differences in age and gestational age(P>0.05),but there were significant differences in neonatal weight,placental weight and Apgar score at 1min and 5min(P<0.05).The expression of A20 was significantly lower in LWB group(P<0.01).Immunohistochemistry showed that A20 was decreased in placental tissue of LBW group,and it was positively correlated with neonatal weight and placental weight(P<0.05).In addition,compared with NC group,the protein expression of A20 in LPS group was significantly decreased(P<0.05),while the protein expression of P-p65,TNF-αand IL-1βin LPS alone group was significantly decreased(P<0.05).After overexpression of A20 gene,the expression of A20 was increased(P<0.05),while the e

关 键 词：锌指蛋白A20 低出生体重儿 新生儿 滋养细胞 炎症 

分 类 号：R722[医药卫生—儿科]