Insights into the mechanisms underlying the biodegradation of phenanthrene in biochar-amended soil:from bioavailability to soil microbial communities  

作　　者：Meng Zhang Yaqi Luo Yitao Zhu Haiyun Zhang Xilong Wang Wei Li Pingping Li Jiangang Han 

机构地区：[1]Co-Innovation Center for Sustainable Forestry in Southern China,College of Biology and the Environment,Nanjing Forestry University,Nanjing 210037,China [2]National Positioning Observation Station of Hung-tse Lake Wetland Ecosystem in Jiangsu Province,Hongze 223100,China [3]Agricultural Environment and Farmland Conservation Experiment Station of Ministry Agriculture,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China [4]Laboratory for Earth Surface Processes,College of Urban and Environmental Sciences,Peking University,Beijing 100871,China

出　　处：《Biochar》2023年第1期273-289,共17页生物炭（英文）

基　　金：National Natural Science Foundation of China(No.42177387,32171628 and 42207436);China Postdoctoral Science Foundation(No.2020M671507);Postdoctoral Research Foundation of Jiangsu Province(No.2021K213B).

摘　　要：The dynamic effect of biochar amendment in contaminated soil on the bioavailability of polycyclic aromatic hydrocarbons(PAHs)and microbial communities and how it comprehensively affects PAH biodegradation remain unclear.This study investigated the effects of wheat straw-derived biochars obtained at 300 and 500℃ at different amendment levels(0.03%and 0.3%)on the mineralization kinetics of phenanthrene with different initial concentrations(2 and 20 mg kg^(−1))in soil by indigenous microorganisms.The results revealed that the addition of biochar inhibited both the rates and extents of mineralization in low-concentration phenanthrene-contaminated soil(PLS)by 38.9-78.3%and 23.9-53.6%,respectively.This was because biochar amendment in the PLS greatly reduced the bioavailable fraction of phenanthrene for degradation owing to its strong sorption and also decreased that to specific degrading bacterial genera,which hindered their growth and reduced their abundances by 1.37-36.6%.However,biochar addition into the soil contaminated with high concentrations of phenanthrene(PHS)resulted in its effective mineralization and enhanced mineralization rates and extents at high amendment levels by 32.4-86.7%and 32.0-44.7%,respectively.This was because biochar amendment in the PHS significantly promoted the abundances of the total bacterial communities(29.9-80.4%)and potential degrading genera(1.89-25.9%)by providing nutrients and stimulated the specific PAH-degradative nidA gene abundance by 1-2 times.These findings will guide the use of biochar to remediate soils with different PAH pollution levels based on the two roles that they play(i.e.,immobilizing PAHs or facilitating PAH degradation).

关 键 词：BIOCHAR PAHs Microbial availability Soil microbial communities MINERALIZATION 

分 类 号：S15[农业科学—土壤学] X53[农业科学—农业基础科学]