Sustained and efficient remediation of biochar immobilized with Sphingobium abikonense on phenanthrene-copper co-contaminated soil and microbial preferences of the bacteria colonized in biochar  

作　　者：Ying Zhang Shuai Liu Lili Niu Aoxue Su Mingyue Li Yuqing Wang Yan Xu 

机构地区：[1]College of Environmental Sciences and Engineering,Qingdao University,Qingdao 266071,China [2]Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province,Hangzhou,China [3]College of Environment and Safety Engineering,Qingdao University of Science and Technology,Qingdao 266042,China [4]Key Laboratory of Pollution Exposure and Health Intervention Technology of Zhejiang Province,Interdisciplinary Research Academy(IRA),Zhejiang Shuren University,Hangzhou,China

出　　处：《Biochar》2023年第1期747-764,共18页生物炭（英文）

基　　金：Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province(20230005);Qingdao Demonstration and Guidance Project of Science and Technology to Benefit the People(21-1-4-sf-17-nsh);National Natural Science Foundation of China(42107419,51878363,42177028);Natural Science Foundation of Shandong Province(ZR2019YQ18);Science and Technology Support Plan for Youth Innovation of Colleges in Shandong Province(DC2000000961);National College Students Innovation and Entrepreneurship Training Program of China(S20211106514).

摘　　要：Immobilized microbial technology has been widely used in wastewater treatment,but it has been used less frequently for soil remediation,particularly in sites that are co-contaminated with organic compounds and heavy metals.In addition,there is limited knowledge on the efficiency of remediation and microbial preferences to colonize the immobilized carriers.In this study,biochar immobilized with Sphingobium abikonense was introduced to remediate soils that were co-contaminated with phenanthrene(PHE)and copper(Cu),and the mechanisms of microbial assemblage were investigated.The immobilized microbial biochar maintained a degradation rate of more than 96%in both the first(0-6 d)and second(6-12 d)contamination periods.The addition of biochar increased the proportion of Cu bound to organic matter,and Fe-Mn oxide bound Cu in the soil.In addition,both Cu and PHE could be adsorbed into biochar pellets in the presence or absence of immobilized S.abikonense.The presence of biochar significantly increased the abundance of bacteria,such as Luteibacter,Bordetella and Dyella,that could degrade organic matter and tolerate heavy metals.Notably,the biochar could specifically select host microbes from the soil for colonization,while the presence of S.abikonense affected this preference.The autonomous selection facilitates the degradation of PHE and/or the immobilization of Cu in the soil.These results provide a green approach to efficiently and sustainably remediate soil co-contaminated with PHE and Cu and highlight the importance of microbial preference colonized in immobilized carriers.

关 键 词：CO-CONTAMINATION Immobilized bacteria Microbial selection BIOCHAR Microbial communities 

分 类 号：S15[农业科学—土壤学] X53[农业科学—农业基础科学]