Biochar reduces colloidal phosphorus in leachate by regulating phoD- and phoC-harboring microbial communities during drying/rewetting cycles  

作　　者：Xiaochun Wang Hongnuo Ge Yunying Fang Chunlong Liu Kamel M.Eltohamy Zekai Wang Xinqiang Liang 

机构地区：[1]Key Laboratory of Watershed Non-point Source Pollution Control and Water Eco-security of Ministry of Water Resources,College of Environmental and Resource Sciences,Zhejiang University,Hangzhou 310058,China [2]College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310058,China [3]Australian Rivers Institute,School of Environment and Science,Griffith University,Nathan Campus,Brisbane,QLD 4111,Australia [4]Key Laboratory of Mollisols Agroecology,Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences,Harbin 150081,China

出　　处：《Biochar》2023年第1期1014-1028,共15页生物炭（英文）

基　　金：National Natural Science Foundation of China(42277005,22076163);Key Research and Development Project of Science and Technology Department of Zhejiang Province(2023C02016,2023C02019);Bingtuan Science and Technology Program(2021DB019).

摘　　要：Drying and rewetting(DRW)events cause the release of colloidal phosphorus(P_(coll),1-1000 nm)in leachate,and biochar is considered an effective inhibitor;however,the microbial mechanism remains elusive.In this study,three successive DRW cycles were performed on the soil columns to assess the effect of biochar addition on P_(coll) content and its possible associates,including phosphatase-producing microbial populations(phoD- and phoC-harboring microbial communities)and alkaline/acid phosphatase(ALP/ACP)activities.Results showed that the biochar addition significantly decreased the P_(coll) by 15.5-32.1%during three DRW cycles.The structural equation model(SEM)confirmed that biochar addition increased phoD- and phoC-harboring microbial communities and ALP/ACP activities,which reduces the release of P_(coll) into leachate.In addition,the manure biochar was more effective than the straw biochar in promoting competition and cooperation in the co-occurrence network(2-5%nodes increased on average),and the key taxa Proteobacteria and Cyanobacteria were identified as the dominant species of potential ALP/ACP activities and P_(coll) content.Our findings provide a novel understanding of biochar reducing P_(coll) loss from the phosphatase perspective by regulating the phoD-and phoC-harboring communities during DRW events.

关 键 词：Drying and rewetting events Alkaline/acid phosphatase Microbial mechanism PROTEOBACTERIA CYANOBACTERIA 

分 类 号：S154.3[农业科学—土壤学]