通关藤水煎液中抗骨肉瘤细胞活性成分研究  

Active Constituents against Osteosarcoma Cells in Marsdeniae Tenacissimae Caulis Decoction

作　　者：吴子薇 李晓波[1] 何越洲 臧志明 董以晨 叶富霖 陈君君 王梦月[1] 韩永龙 WU Ziwei;LI Xiaobo;HE Yuezhou;ZANG Zhiming;DONG Yichen;YE Fulin;CHEN Junjun;WANG Mengyue;HAN Yonglong(School of Pharmaceutical Sciences,Shanghai Jiao Tong University,Shanghai 200240,China;Department of Pharmacy,Shanghai Jiao Tong University Affiliated Sixth People′s Hospital,Shanghai 200233,China)

机构地区：[1]上海交通大学药学院,上海200240 [2]上海交通大学医学院附属第六人民医院药剂科,上海200233

出　　处：《世界中医药》2024年第16期2378-2386,共9页World Chinese Medicine

基　　金：国家自然科学基金项目(82003987);上海市浦东新区卫健委科技项目卫生行业专项(PW2021E-03);上海市浦东新区科技发展基金民生科研专项(PKJ2023-Y51);内蒙古自治区“科技兴蒙”计划项目(2022XYJG0001-02-01)。

摘　　要：目的:研究通关藤水煎液中抗骨肉瘤细胞活性成分。方法:采用大孔树脂柱层析、硅胶柱层析、制备型高效液相色谱(HPLC)、结合磁共振成像波谱(NMR)、高分辨率质谱(HR-MS)等方法,对通关藤水煎液的化学成分进行系统分离与结构鉴定;采用细胞计数检测(CCK-8)方法进行抗骨肉瘤细胞活性评价。结果:分离鉴定了33个化合物。其中,化合物1~8、化合物24~28均为首次从通关藤中分得;化合物6、化合物9、化合物21、化合物22、化合物25、化合物26、化合物29~31均能显著抑制骨肉瘤细胞MG63、143B的增殖。结论:通关藤水煎液中多个成分具有抑制骨肉瘤细胞活性。研究结果为通关藤的应用及进一步开发提供了科学依据。Objective:To investigate the active constituents against osteosarcoma cells in Marsdeniae Tenacissimae Caulis(MTC)decoction.Methods:The chemical constituents in MTC decoction were investigated by macroporous resin column chromatography(CC),silica gel CC,preparative high performance liquid chromatography(HPLC),nuclear magnetic resonance(NMR)spectroscopy,and high-resolution mass spectroscopy(HR-MS).Their anti-osteosarcoma activities were evaluated by the CCK-8 method.Results:Thirty-three compounds were isolated and identified.Among them,compounds 1 to 8 and 24 to 28 were isolated from Marsdeniae Tenacissimae Caulis for the first time.Compounds 6,9,21,22,25,26,and 29 to 31 could significantly inhibit the proliferation of osteosarcoma cells MG63 and 143B.Conclusion:Multiple components of MTC decoction had the inhibitory activities against osteosarcoma cells.This study provides a scientific basis for the application and further exploitation of MTC.

关 键 词：通关藤 水煎液 骨肉瘤 活性成分 

分 类 号：R285[医药卫生—中药学]