海洋多糖对葡聚糖硫酸钠诱导的溃疡性肠炎小鼠的改善作用  

Ameliorative Effects of Marine Polysaccharides on DSS-induced Ulcerative Colitis in Mice

作　　者：黄蓉 曲晨 王润 童桦 袁煜萱 任安琪 刘志海 李秋 HUANG Rong;QU Chen;WANG Run;TONG Hua;YUAN Yuxuan;REN Anqi;LIU Zhihai;LI Qiu(Qingdao Agricultural University,Qingdao 266000,China)

机构地区：[1]青岛农业大学,青岛266000

出　　处：《世界中医药》2024年第16期2427-2432,共6页World Chinese Medicine

基　　金：国家自然科学基金项目(82003922);山东省青创科技团队项目(2021KJ094)。

摘　　要：目的:研究海洋中药多糖海藻酸钠和褐藻多糖对葡聚糖硫酸钠(DSS)诱导的小鼠溃疡性结肠炎(UC)的改善作用。方法:使用小鼠单核巨噬细胞白血病(RAW264.7)细胞系作为体外实验的细胞模型,将不同浓度的海藻酸钠和褐藻多糖作用于RAW264.7细胞,通过细胞计数(CCK-8)法测定细胞活力;并用脂多糖(LPS)诱导RAW264.7细胞激活,通过酶联免疫吸附试验(ELISA)试剂盒测定细胞上清液中的一氧化氮(NO)、白细胞介素-6(IL-6)含量;将25只雌性小鼠随机分为空白对照组、模型组、阳性对照组、海藻酸钠及褐藻多糖组,每组5只。除空白对照组外,每组使用DSS灌胃7 d以建立UC模型,之后,对阳性对照组、海藻酸钠组及褐藻多糖组小鼠连续灌胃给药(后生元、海藻酸钠和褐藻多糖)。眼球取血用于血清中炎症介质的检测;同时,麻醉处死小鼠取小鼠结肠组织,用于生化指标测定和组织病理学分析。结果:海藻酸钠和褐藻多糖在高浓度也未对RAW264.7细胞造成毒性;海藻酸钠、褐藻多糖在500~1000μg/mL浓度范围内,都以浓度依赖性抑制RAW264.7细胞分泌细胞因子NO和IL-6,且褐藻多糖效果明显优于海藻酸钠;对于DSS诱导的小鼠UC,海藻酸钠、褐藻多糖均可改善UC的临床症状,使小鼠体质量回升,降低血清炎症介质肿瘤坏死因子-α(TNF-α)和IL-6的分泌水平。结论:褐藻多糖和海藻酸钠能够通过抑制血清中炎症介质的释放来治疗DSS诱导的UC,且褐藻多糖的治疗效果优于海藻酸钠。Objective:To investigate the effects of polysaccharides(sodium alginate and fucoidan)from marine Chinese medicines on dextran sulfate sodium(DSS)-induced ulcerative colitis(UC)in mice.Methods:RAW264.7 cells were treated with different concentrations of sodium alginate and fucoidan,and cell viability was measured by CCK-8 assay.RAW264.7 cells were activated by lipopolysaccharide(LPS).ELISA kits were used to determine the content of NO and interleukin-6(IL-6)in the cell supernatant.Twenty-five female mice were randomly divided into blank control group,model group,positive control group,sodium alginate group,and fucoidan group,with 5 mice in each group.Except the blank control group,each group was treated with DSS by gavage for 7 days to establish a US model,and then the positive control group,the sodium alginate group,and the fucoidan group were treated with postbiotics,sodium alginate,and fucoidan by gavage,respectively.Blood samples were collected from eyeballs for the detection of serum inflammatory mediators.At the same time,mice were sacrificed under anesthesia,and colon tissues were obtained for biochemical indexes determination and histopathological analysis.Results:Sodium alginate and fucoidan did not cause toxicity to RAW264.7 cells even at high concentrations.In the concentration range of 500 to 1000μg/mL,both sodium alginate and fucoidan inhibited the secretion of NO and IL-6 in RAW264.7 cells in a concentration-dependent manner,and the effect of fucoidan was significantly better than that of sodium alginate.For DSS-induced UC in mice,sodium alginate and fucoidan can improve the clinical symptoms of UC,restore the body weight of mice,and reduce the secretion levels of serum inflammatory mediators tumor necrosis factor-α(TNF-α)and IL-6.Conclusion:Fucoidan and sodium alginate can treat DSS-induced UC by inhibiting the release of inflammatory mediators in serum,and the therapeutic effect of fucoidan is better than that of sodium alginate.

关 键 词：海洋中药多糖 海藻酸钠 褐藻多糖 溃疡性肠炎 脂多糖 炎症介质 葡聚糖硫酸钠 RAW264.7细胞 

分 类 号：R285[医药卫生—中药学]