Integrated printed BDNF-stimulated HUCMSCs-derived exosomes/collagen/chitosan biological scaffolds with 3D printing technology promoted the remodelling of neural networks after traumatic brain injury  

作　　者：Xiaoyin Liu Jian Zhang Xu Cheng Peng Liu Qingbo Feng Shan Wang Yuanyou Li Haoran Gu Lin Zhong Miao Chen Liangxue Zhou 

机构地区：[1]Department of Neurosurgery,West China Hospital,West China Medical School,Sichuan University,Chengdu,Sichuan 610041,China [2]National Engineering Research Center for Biomaterials,College of Biomedical Engineering,Sichuan University,Chengdu,Sichuan 610064,China [3]Tianjin Key Laboratory of Neurotrauma Repair,Institute of Traumatic Brain Injury and Neuroscience,Characteristic Medical Center of Chinese People’s Armed Police Force,Tianjin 300162,China [4]Department of Anesthesiology,West China Hospital,Sichuan University,Chengdu,Sichuan 610064,China [5]Department of Liver Surgery&Liver Transplantation,State Key Laboratory of Biotherapy and Cancer Center,West China Hospital,Sichuan University,Chengdu,Sichuan 610041,China [6]The 947th Hospital of Chinese People’s Liberation Army,Xinjiang Uygur Autonomous Region,Kashgar 844000,China [7]The First Affiliated Hospital of Chengdu Medical College,Chengdu,Sichuan 610500,China [8]Intensive Care Unit,Traditional Chinese Medicine Hospital of Xinjiang Uyghur Autonomous Region and Affiliated Hospital of Traditional Chinese Medicine of Xinjiang Medical University,Xinjiang Uygur Autonomous Region,Urumqi 830000,China

出　　处：《Regenerative Biomaterials》2023年第1期40-52,共13页再生生物材料（英文版）

基　　金：supported by the National Major Scientific and Technological Special Project for Significant New Drugs Development(2015ZX09102010).

摘　　要：The restoration of nerve dysfunction after traumatic brain injury(TBI)faces huge challenges due to the limited self-regenerative abilities of nerve tissues.In situ inductive recovery can be achieved utilizing biological scaffolds combined with endogenous human umbilical cord mesenchymal stem cells(HUCMSCs)-derived exosomes(MExos).In this study,brain-derived neurotrophic factor-stimulated HUCMSCs-derived exosomes(BMExos)were composited with collagen/chitosan by 3D printing technology.3D-printed collagen/chitosan/BMExos(3D-CC-BMExos)scaffolds have excellent mechanical properties and biocompatibility.Subsequently,in vivo experiments showed that 3D-CC-BMExos therapy could improve the recovery of neuromotor function and cognitive function in a TBI model in rats.Consistent with the behavioural recovery,the results of histomorphological tests showed that 3D-CC-BMExos therapy could facilitate the remodelling of neural networks,such as improving the regeneration of nerve fibres,synaptic connections and myelin sheaths,in lesions after TBI.

关 键 词：collagen CHITOSAN BDNF exosomes mesenchymal stem cell traumatic brain injury 

分 类 号：R318[医药卫生—生物医学工程] TP391.73[医药卫生—基础医学]