Injectable and biofunctionalized fibrin hydrogels co-embedded with stem cells induce hair follicle genesis  

作　　者：Haiyan Chen Xiaoxiao Ma Mengqi Zhang Zhonghua Liu 

机构地区：[1]The National and Local Joint Engineering Laboratory of Animal Peptide Drug Development,College of Life Sciences,Hunan Normal University,Changsha 410081,People’s Republic of China [2]Tsinghua Shenzhen International Graduate School,Tsinghua University,Shenzhen 518055,People’s Republic of China

出　　处：《Regenerative Biomaterials》2023年第1期53-63,共11页再生生物材料（英文版）

基　　金：supported by National Natural Science Foundation of China(32000956);the Science and Technology Innovation Program of Hunan Province(2020RC4023).

摘　　要：Fibrin-based hydrogels have been widely used in various tissue engineering because of their biocompatibility,biodegradability,tunable mechanical characteristics and nanofibrous structural properties.However,their ability to support stem cells for hair follicle neogenesis is unclear.In this study,we investigated the effect of fibrin hydrogels in supporting skin-derived precursors(SKPs)in hair follicle neogenesis.Our results showed that SKPs in fibrin hydrogels with high cell viability and proliferation,the stemness of SKPs could be maintained,and the expression of hair induction signature genes such as akp2 and nestin was enhanced.Moreover,hair follicle reconstruction experiments showed de novo hair genesis in mice and the hairs persisted for a long time without teratoma formation.More importantly,the blood vessels and sebaceous glands were also regenerated.Our study demonstrated that fibrin hydrogels are promising in hair follicle regeneration and have potential application in clinical settings for alopecia and wound healing.

关 键 词：fibrin hydrogels FIBRINOGEN hair follicle neogenesis ALOPECIA skin-derived precursors 

分 类 号：TQ427.26[化学工程]