Development of tropoelastin-functionalized anisotropic PCL scaffolds for musculoskeletal tissue engineering  

作　　者：Miao Zhang Ziyu Wang Anyu Zhang Linyang Liu Suzanne M.Mithieux Marcela M.M.Bilek Anthony S.Weiss 

机构地区：[1]Charles Perkins Centre,The University of Sydney,Sydney,NSW 2006,Australia [2]School of Life and Environmental Sciences,The University of Sydney,Sydney,NSW 2006,Australia [3]Applied and Plasma Physics Laboratory,School of Physics,The University of Sydney,Sydney,NSW 2006,Australia [4]School of Biomedical Engineering,The University of Sydney,Sydney,NSW 2006,Australia [5]Sydney Nano Institute,The University of Sydney,Sydney,NSW 2006,Australia

出　　处：《Regenerative Biomaterials》2023年第1期64-72,共9页再生生物材料（英文版）

基　　金：supported by an Australian Commonwealth Government Research Training Program Tuition Fee Offset and Stipend Scholarship.A.S.W.acknowledges funding from the National Health and Medical Research Council(APP1195827);M.M.M.B.acknowledges funding from the Australian Research Council(FL190100216).

摘　　要：The highly organized extracellular matrix(ECM)of musculoskeletal tissues,encompassing tendons,ligaments and muscles,is structurally anisotropic,hierarchical and multi-compartmental.These features collectively contribute to their unique function.Previous studies have investigated the effect of tissue-engineered scaffold anisotropy on cell morphology and organization for musculoskeletal tissue repair and regeneration,but the hierarchical arrangement of ECM and compartmentalization are not typically replicated.Here,we present a method for multi-compartmental scaffold design that allows for physical mimicry of the spatial architecture of musculoskeletal tissue in regenerative medicine.This design is based on an ECM-inspired macromolecule scaffold.Polycaprolactone(PCL)scaffolds were fabricated with aligned fibers by electrospinning and mechanical stretching,and then surface-functionalized with the cell-supporting ECM protein molecule,tropoelastin(TE).TE was attached using two alternative methods that allowed for either physisorption or covalent attachment,where the latter was achieved by plasma ion immersion implantation(PIII).Aligned fibers stimulated cell elongation and improved cell alignment,in contrast to randomly oriented fibers.TE coatings bound by physisorption or covalently following 200 s PIII treatment promoted fibroblast proliferation.This represents the first cytocompatibility assessment of novel PIII-treated TE-coated PCL scaffolds.To demonstrate their versatility,these 2D anisotropic PCL scaffolds were assembled into 3D hierarchical constructs with an internally compartmentalized structure to mimic the structure of musculoskeletal tissue.

关 键 词：TROPOELASTIN PCL musculoskeletal tissue engineering plasma immersion ion implantation ELECTROSPINNING anisotropy hierarchical 

分 类 号：R318[医药卫生—生物医学工程]