Micropatterned composite membrane guides oriented cell growth and vascularization for accelerating wound healing  

作　　者：Jiaqi Li Xulong Liu Weiyong Tao Yan Li Yingying Du Shengmin Zhang 

机构地区：[1]Advanced Biomaterials and Tissue Engineering Center,Huazhong University of Science and Technology,Wuhan 430074,China [2]NMPA Research Base of Regulatory Science for Medical Devices&Institute of Regulatory Science for Medical Devices,Huazhong University of Science and Technology,Wuhan 430074,China [3]Institute of Biomaterials and Medical Devices,Wuhan Institute of Industrial Innovation and Development,Wuhan 430074,China [4]Department of Biomedical Engineering,Huazhong University of Science and Technology,Wuhan 430074,China

出　　处：《Regenerative Biomaterials》2023年第1期344-353,共10页再生生物材料（英文版）

基　　金：supported by the National Natural Science Foundation of China(31870960,32171331 and 82130061);the Fundamental Research Funds for the Central Universities,Huazhong University of Science and Technology(2019kfyXMBZ021 and 2020kfyXJJS115).

摘　　要：Skin defect is common in daily life,but repairing large skin defects remains a challenge.Using biomaterials to deliver biochemical or physical factors to promote skin tissue regeneration is of great significance for accelerating wound healing.Specific surface micropatterns on biomaterials could affect cell behavior and tissue regeneration.However,few studies have focused on the construction of wound healing biomaterials with surface micropatterns and their role in skin tissue regeneration.In the present study,gelatin-polycaprolactone/silk fibroin composite membranes with different micropatterns were fabricated by photolithography,including line,grid and plane micropatterns.In vitro cell experiments demonstrated that the line micropattern on the composite membrane could guide cell-oriented growth,and more importantly,promote the expression of angiogenesis-related markers andα-smooth muscle actin(α-SMA)at both gene level and protein level.In the rat full-thickness skin defect model,the composite membrane with line micropatterns increasedα-SMA production and neovascularization in wounds,leading to accelerated wound contraction and healing.The current study not only suggests that composite membranes with specific micropatterns can be promising wound repair materials but also provides new insights into the importance of biomaterial surface topology for tissue regeneration.

关 键 词：TOPOLOGY cell behavior wound healing GELATIN polymer biomaterials 

分 类 号：R318[医药卫生—生物医学工程]