Age-related changes in the ratio of Type I/III collagen and fibril diameter in mouse skin  

作　　者：Jianping Gao Zhenhu Guo Yang Zhang Yuying Liu Fangyu Xing Junjie Wang Xi Luo Yingjun Kong Guifeng Zhang 

机构地区：[1]National Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100190,China [2]School of Chemical and Engineering,University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Regenerative Biomaterials》2023年第1期377-385,共9页再生生物材料（英文版）

基　　金：supported by the National Key Technology R&D Programs of China(2021YFC2400800);Science and Technology Program of Guangzhou,China(201803010086);Open Funding Project of the State Key Laboratory of Biochemical Engineering(2021KF-04);Independent Research Project of the State Key Laboratory of Biochemical Engineering(2021ZZ-03).

摘　　要：The content of type I collagen(COL-I)and type III collagen(COL-III)and the ratio between them not only affect the skin elasticity and mechanical strength,but also determine the fibril diameter.In this research,we investigated the age-related changes in COL-I/COL-III ratio with their formed fibril diameter.The experimental result was obtained from high performance liquid chromatography-mass spectrometer,hydroxyproline determination,picrosirius red staining and transmission electron microscopes(TEM),respectively.The result indicated that the COL-I/COL-III ratio in mouse skin increased with aging.From the 0th to 9th week,the COL-I/COLIII ratio increased from 1.3:1 to 4.5:1.From the 9th to the 18th week,it remained between 4.5:1 and 4.9:1.The total content of COL-I and COL-III firstly increased and then decreased with aging.The TEM result showed that the fibril diameter increased with aging.From the 0th to 9th week,the average fibril diameter increased from 40 to 112 nm;From the 9th to 18th weeks,it increased from 112 to 140 nm.After the 9th week.The fibril diameter showed obvious uneven distribution.Thus,the COL-I/COLIII ratio was proportional to the fibril diameter,but inversely proportional to the uniformity of fibril diameter.

关 键 词：COLLAGEN SKIN RATIO fibril diameter 

分 类 号：R318.08[医药卫生—生物医学工程]