Biological properties of self-assembled nanofibers of elastin-like block polypeptides for tissue-engineered vascular grafts:platelet inhibition,endothelial cell activation and smooth muscle cell maintenance  

作　　者：Kazuki Natsume Jin Nakamura Kazuhide Sato Chikara Ohtsuki Ayae Sugawara-Narutaki 

机构地区：[1]Department of Materials Chemistry,Graduate School of Engineering,Nagoya University,Nagoya 464-8603,Japan [2]Department of Biological Functions Engineering,Graduate School of Life Science and Systems Engineering,Kyushu Institute of Technology,Fukuoka 808-0196,Japan [3]Institute for Advanced Research,Nagoya University,Nagoya 464-8601,Japan [4]Department of Respiratory Medicine,Graduate School of Medicine,Nagoya University,Nagoya 466-8560,Japan [5]Department of Energy Engineering,Graduate School of Engineering,Nagoya University,Nagoya 464-8603,Japan

出　　处：《Regenerative Biomaterials》2023年第1期386-395,共10页再生生物材料（英文版）

基　　金：supported by the Ministry of Education,Culture,Sports,Science and Technology(MEXT)Program:Data Creation and Utilization-Type Material Research and Development Project[JPMXP1122714694];Japan Society for the Promotion of Science(JSPS)KAKENHI[19H03747];Japan Agency for Medical Research and Development(AMED)[JP20lm0203005];a Research Grant from Japan Association for Chemical Innovation(JACI).

摘　　要：Strategic materials design is essential for the development of small-diameter,tissue-engineered vascular grafts.Self-assembled nanofibers of elastin-like polypeptides represent promising vascular graft components as they replicate the organized elastin structure of native blood vessels.Further,the bioactivity of nanofibers can be modified by the addition of functional peptide motifs.In the present study,we describe the development of a novel nanofiber-forming elastin-like polypeptide(ELP)with an arginine-glutamic acid-aspartic acid-valine(REDV)sequence.The biological characteristics of the REDV-modified ELP nanofibers relevant to applications in vascular grafting were compared to ELP without ligands for integrin,ELP with arginine-glycine-aspartic acid(RGD)sequence,collagen and cell culture glass.Among them,REDV-modified ELP nanofibers met the preferred biological properties for vascular graft materials,i.e.(i)inhibition of platelet adhesion and activation,(ii)endothelial cell adhesion and proliferation and(iii)maintenance of smooth muscle cells in a contractile phenotype to prevent cell overgrowth.The results indicate that REDV-modified ELP nanofibers represent promising candidates for the further development of small-diameter vascular grafts.

关 键 词：elastin-like polypeptide NANOFIBER PLATELET endothelial cell smooth muscle cell 

分 类 号：R318[医药卫生—生物医学工程]