Bioactive fibrous scaffolds with programmable release of polypeptides regulate inflammation and extracellular matrix remodeling  

作　　者：Zehong Xiang Xinghua Guan Zhifang Ma Qiang Shi Mikhail Panteleev Fazly I.Ataullakhanov 

机构地区：[1]State Key Laboratory of Polymer Physics and Chemistry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun,Jilin 130022,China [2]University of Science and Technology of China,Hefei,Anhui 230026,China [3]Key Laboratory of Polymeric Materials Design and Synthesis for Biomedical Function,Soochow University,Suzhou 215123,China [4]Dmitry Rogachev National Research Center of Pediatric Hematology,Oncology and Immunology,Moscow 117198,Russia [5]Faculty of Physics,Lomonosov Moscow State University,Moscow 119991,Russia

出　　处：《Regenerative Biomaterials》2023年第1期522-532,共11页再生生物材料（英文版）

基　　金：supported by the financial support of the National Natural Science Foundation of China(52061135202,51573186).

摘　　要：Inflammation manipulation and extracellular matrix(ECM)remodeling for healthy tissue regeneration are critical requirements for tissue engineering scaffolds.To this end,the bioactive polycaprolactone(PCL)-based scaffolds are fabricated to release aprotinin and thymosinβ4(Tβ4)in a programmable manner.The core part of the fiber is composed of hyaluronic acid and Tβ4,and the shell is PCL,which is further coated with heparin/gelatin/aprotinin to enhance biocompatibility.The in vitro assay demonstrates that the controlled release of aprotinin prevents initial excessive inflammation.The subsequent release of Tβ4 after 3 days induces the transition of macrophages from M1 into M2 polarization.The manipulation of inflammatory response further controls the expression of transforming growth factor-βand fibroblast activation,which oversee the quantity and quality of ECM remodeling.In addition,the gradual degradation of the scaffold allows cells to proliferate within the platform.In vivo implant evaluation convinces that PCL-based scaffolds possess the high capability to control the inflammatory response and restore the ECM to normal conditions.Hence,our work paves a new way to develop tissue engineering scaffolds for inflammation manipulation and ECM remodeling with peptide-mediated reactions.

关 键 词：bioactive scaffolds inflammation control extracellular matrix remodeling polypeptide release electrospinning 

分 类 号：R318[医药卫生—生物医学工程]