Kinetically inert manganese(II)-based hybrid micellar complexes for magnetic resonance imaging of lymph node metastasis  

作　　者：Kai Chen Zhongyuan Cai Yingzi Cao Lingling Jiang Yuting Jiang Haojie Gu Shengxiang Fu Chunchao Xia Su Lui QiyongGong Bin Song Hua Ai 

机构地区：[1]National Engineering Research Center for Biomaterials,Sichuan University,Chengdu 610064,China [2]Department of Radiology,West China Hospital,Sichuan University,Chengdu 610041,China [3]Huaxi MR Research Center(HMRRC),Department of Radiology,Frontiers Science Center for Disease-Related Molecular Network,State Key Laboratory of Biotherapy,West China Hospital,Sichuan University,Chengdu 610041,China [4]Functional and Molecular Imaging Key Laboratory of Sichuan Province,Key Laboratory of Transplant Engineering and Immunology,NHC,Research Unit of Psychoradiology,Chinese Academy of Medical Sciences,Chengdu 610041,China [5]Department of Radiology,West China Xiamen Hospital of Sichuan University,Fujian,Xiamen 361000,China [6]Department of Radiology,Sanya People’s Hospital,Sanya 572000,China

出　　处：《Regenerative Biomaterials》2023年第1期1122-1134,共13页再生生物材料（英文版）

基　　金：supported by the National Natural Science Foundation of China(NSFC,52073192);the Innovative Research Groups of the National Natural Science Foundation of China(81621003).

摘　　要：The localization and differential diagnosis of the sentinel lymph nodes(SLNs)are particularly important for tumor staging,surgical planning and prognosis.In this work,kinetically inert manganese(II)-based hybrid micellar complexes(MnCs)for magnetic resonance imaging(MRI)were developed using an amphiphilic manganese-based chelate(C18-PhDTA-Mn)with reliable kinetic stability and self-assembled with a series of amphiphilic PEG-C18 polymers of different molecular weights(C18En,n=10,20,50).Among them,the probes composed by 1:10 mass ratio of manganese chelate/C18En had slightly different hydrodynamic particle sizes with similar surface charges as well as considerable relaxivities(∼13 mM^(−1)s^(−1)at 1.5 T).In vivo lymph node imaging in mice revealed that the MnC MnC-20 formed by C18E20 with C18-PhDTA-Mn at a hydrodynamic particle size of 5.5 nm had significant signal intensity brightening effect and shortened T1 relaxation time.At an imaging probe dosage of 125μg Mn/kg,lymph nodes still had significant signal enhancement in 2 h,while there is no obvious signal intensity alteration in non-lymphoid regions.In 4T1 tumor metastatic mice model,SLNs showed less signal enhancement and smaller T1 relaxation time variation at 30 min post-injection,when compared with normal lymph nodes.This was favorable to differentiate normal lymph nodes from SLN under a 3.0-T clinical MRI scanner.In conclusion,the strategy of developing manganese-based MR nanoprobes was useful in lymph node imaging.

关 键 词：kinetically inert amphiphilic manganese chelate T1 contrast agent sentinel lymph node magnetic resonance imaging 

分 类 号：R318[医药卫生—生物医学工程]