Chitin nanocrystal-assisted 3D bioprinting of gelatin methacrylate scaffolds  

作　　者：Zhengyun Ling Jian Zhao Shiyu Song Shuwei Xiao Pengchao Wang Ziyan An Zhouyang Fu Jinpeng Shao Zhuang Zhang Weijun Fu Shenghan Song 

机构地区：[1]School of Medicine,Nankai University,Tianjin 300071,China [2]Department of Urology,The Third Medical Center,PLA General Hospital,Beijing 100039,China [3]Medical School of PLA,Beijing 100853,China [4]Department of Urology,960th Hospital of PLA,Jinan 250031,China [5]Undergraduate Student Majoring in Clinical Pharmacy,Chongqing Medical University,Chongqing 400016,China [6]Department of Urology,Air Force Medical Center,Beijing 100142,China [7]Beijing Institute of Basic Medical Sciences,Beijing 100850,China [8]Department of Vascular Surgery,Beijing Chaoyang Hospital,Capital Medical University,Beijing 100020,China

出　　处：《Regenerative Biomaterials》2023年第1期1192-1201,共10页再生生物材料（英文版）

基　　金：supported by the National Natural Science Foundation of China(82270721);the National Natural Science Foundation of China(81873600).

摘　　要：In recent years,there has been an increasing focus on the application of hydrogels in tissue engineering.The integration of 3D bioprinting technology has expanded the potential applications of hydrogels.However,few commercially available hydrogels used for 3D biological printing exhibit both excellent biocompatibility and mechanical properties.Gelatin methacrylate(GelMA)has good biocompatibility and is widely used in 3D bioprinting.However,its low mechanical properties limit its use as a standalone bioink for 3D bioprinting.In this work,we designed a biomaterial ink composed of GelMA and chitin nanocrystal(ChiNC).We explored fundamental printing properties of composite bioinks,including rheological properties,porosity,equilibrium swelling rate,mechanical properties,biocompatibility,effects on the secretion of angiogenic factors and fidelity of 3D bioprinting.The results showed that adding 1%(w/v)ChiNC to 10%(w/v)GelMA improved the mechanical properties and printability of the GelMA hydrogels,promoted cell adhesion,proliferation and vascularization and enabled the printing of complex 3D scaffolds.This strategy of incorporating ChiNC to enhance the performance of GelMA biomaterials could potentially be applied to other biomaterials,thereby expanding the range of materials available for use.Furthermore,in combination with 3D bioprinting technology,this approach could be leveraged to bioprint scaffolds with complex structures,further broadening the potential applications in tissue engineering.

关 键 词：3D bioprinting gelatin methacrylate chitin nanocrystal HYDROGELS scaffolds 

分 类 号：R318[医药卫生—生物医学工程] TP391.73[医药卫生—基础医学]