A controllable gelatin-based microcarriers fabrication system for the whole procedures of MSCs amplification and tissue engineering  

作　　者：Zixian Wang Xiuxiu Zhang Limin Xue Gangwei Wang Xinda Li Jianwei Chen Ruxiang Xu Tao Xu 

机构地区：[1]Precision Medicine and Healthcare Research Center,Tsinghua-Berkeley Shenzhen Institute(TBSI),Tsinghua University,Shenzhen 518055,People’s Republic of China [2]Department of Research and Development,Huaqing Zhimei(Shenzhen)Biotechnology Co.,Ltd.,Shenzhen 518107,People’s Republic of China [3]Department of Emergency,The Third Affiliated Hospital,Sun Yat-sen University,Guangzhou 510630,People’s Republic of China [4]Department of Neurosurgery,Sichuan Provincial People’s Hospital,University of Electronic Science and Technology of China,Chengdu 610072,People’s Republic of China [5]Bio-intelligent Manufacturing and Living Matter Bioprinting Center,Research Institute of Tsinghua University in Shenzhen,Tsinghua University,Shenzhen 518057,People’s Republic of China

出　　处：《Regenerative Biomaterials》2023年第1期1303-1313,共11页再生生物材料（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant No.52075285);the Applied Basic Research Project of Sichuan Province(Grant No.2021YJ0563).

摘　　要：Biopolymer microbeads present substantial benefits for cell expansion,tissue engineering,and drug release applications.However,a fabrication system capable of producing homogeneous microspheres with high precision and controllability for cell proliferation,passaging,harvesting and downstream application is limited.Therefore,we developed a co-flow microfluidics-based system for the generation of uniform and size-controllable gelatin-based microcarriers(GMs)for mesenchymal stromal cells(MSCs)expansion and tissue engineering.Our evaluation of GMs revealed superior homogeneity and efficiency of cellular attachment,expansion and harvest,and MSCs expanded on GMs exhibited high viability while retaining differentiation multipotency.Optimization of passaging and harvesting protocols was achieved through the addition of blank GMs and treatment with collagenase,respectively.Furthermore,we demonstrated that MSC-loaded GMs were printable and could serve as building blocks for tissue regeneration scaffolds.These results suggested that our platform held promise for the fabrication of uniform GMs with downstream application of MSC culture,expansion and tissue engineering.

关 键 词：microfluidics size-controllability gelatin-based microcarriers MSCs expansion tissue engineering 

分 类 号：R318[医药卫生—生物医学工程]