Incorporation of kartogenin and silk fibroin scaffolds promotes rat articular cartilage regeneration through enhancement of antioxidant functions  

作　　者：Zejun Pan Mingzhuang Hou Yijian Zhang Yang Liu Xin Tian Xiayu Hu Xiaoyang Ge Zhijian Zhao Tao Liu Yong Xu Huilin Yang Hao Liu Xuesong Zhu Fan He 

机构地区：[1]Department of Orthopaedics,The First Affiliated Hospital of Soochow University,Soochow University,Suzhou 215006,China [2]Orthopaedic Institute,Suzhou Medical College of Soochow University,Soochow University,Suzhou 215007,China [3]Department of Orthopaedics,People’s Hospital of Zhenhai District(Ningbo No.7 Hospital),Ningbo 315202,China

出　　处：《Regenerative Biomaterials》2023年第1期1400-1412,共13页再生生物材料（英文版）

基　　金：supported by the Natural Science Foundation of Jiangsu Province(BK20220046);the National Natural Science Foundation of China(82272494,82072442);the Postgraduate Research&Practice Innovation Program of Jiangsu Province(KYCX21_2971);Key Laboratory of Orthopaedics of Suzhou(SZS2022017);the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).

摘　　要：Treating articular cartilage defects in patients remains a challenging task due to the absence of blood vessels within the cartilage tissue.The regenerative potential is further compromised by an imbalance between anabolism and catabolism,induced by elevated levels of reactive oxygen species.However,the advent of tissue engineering introduces a promising strategy for cartilage regeneration,offering viable solutions such as mechanical support and controlled release of chondrogenic molecules or cytokines.In this study,we developed an antioxidant scaffold by incorporating natural silk fibroin(SF)and kartogenin(KGN)-loaded liposomes(SF-Lipo@KGN).The scaffold demonstrated appropriate pore size,connectivity,and water absorption and the sustained release of KGN was achieved through the encapsulation of liposomes.In vitro experiments revealed that the SF-Lipo@KGN scaffolds exhibited excellent biocompatibility,as evidenced by enhanced cell adhesion,migration,and proliferation of chondrocytes.The SF-Lipo@KGN scaffolds were found to stimulate cartilage matrix synthesis through the activation of the nuclear factor erythroid-2-related factor 2/heme oxygenase-1 antioxidant signaling pathway.In vivo experiments demonstrated the effective promotion of articular cartilage regeneration by the SF-Lipo@KGN scaffolds,which enhanced extracellular matrix anabolism and restored the intrinsic redox homeostasis.Overall,this study successfully developed biomimetic KGN-loaded scaffolds that restore cartilage redox homeostasis,indicating promising prospects for cartilage tissue engineering.

关 键 词：articular cartilage silk fibroin kartogenin extracellular matrix redox balance NRF2 

分 类 号：R318[医药卫生—生物医学工程]