The influence of tag sequence on recombinant humanized collagen(rhCol)and the evaluation of rhCol on Schwann cell behaviors  

作　　者：Mingxuan Bai Ning Kang Yang Xu Jing Wang Xinxing Shuai Caojie Liu Yixuan Jiang Yu Du Ping Gong Hai Lin Xingdong Zhang 

机构地区：[1]State Key Laboratory of Oral Diseases&National Center for Stomatology&National Clinical Research Center for Oral Diseases,West China Hospital of Stomatology,Sichuan University,Chengdu,Sichuan 610041,P.R.China [2]National Engineering Research Center for Biomaterials,College of Biomedical Engineering,Sichuan University,Chengdu,Sichuan 610065,P.R.China

出　　处：《Regenerative Biomaterials》2023年第1期1592-1603,共12页再生生物材料（英文版）

基　　金：sponsored by National Natural Science Foundation of China(Nos 32371399,82170935,32071330).

摘　　要：Recombinant humanized collagen(rhCol)was an extracellular matrix(ECM)-inspired biomimetic biomaterial prepared by biosynthesis technology,which was considered non-allergenic and could possibly activate tissue regeneration.The influence of tag sequence on both structures and performances of rhCol type III(rhCol III)was investigated,and the effect of rhCol III on cell behaviors was evaluated and discussed using Schwann cells(SCs)as in vitro model that was critical in the repair process after peripheral nerve injury.The results demonstrated that the introduction of tag sequence would influence both advanced structures and properties of rhCol III,while rhCol III regulated SCs adhesion,spreading,migration and proliferation.Also,both nerve growth factor and brain-derived neurotrophic factor increased when exposed to rhCol III.As the downstream proteins of integrin-mediated cell adhesions,phosphorylation of focal adhesion kinase and expression of vinculin was up-regulated along with the promotion of SCs adhesion and migration.The current findings contributed to a better knowledge of the interactions between rhCol III and SCs,and further offered a theoretical and experimental foundation for the development of rhCol III-based medical devices and clinical management of peripheral nerve injury.

关 键 词：recombinant humanized collagen Tag sequence peripheral nerve regeneration Schwann cell 

分 类 号：R318[医药卫生—生物医学工程]