满山红配方颗粒提取工艺优化及质量标准研究  

Optimization of extraction process and establishment of quality standard for Folium Rhododendri Daurici formula granules

作　　者：赵奇 房思远 李辰荃 朱永明 徐盼盼 赵兵令 ZHAO Qi;FANG Siyuan;LI Chenquan;ZHU Yongming;XU Panpan;ZHAO Bingling(College of Traditional Chinese Veterinary Medicine,Hebei Agricultural University,Baoding Hebei 071000,China;Hebei Weierli Animal Pharmaceutical Group Company,Shijiazhuang Hebei 050200,China;Hebei Veterinary Medicine Industry Technology Research Institute,Shijiazhuang Hebei 050200,China;Shijiazhuang Institute of Traditional Chinese Medicine for Poultry Industry Technology,Shijiazhuang Hebei 050200,China;Hebei Functional Feed Additives Technology Innovation Center,Shijiazhuang Hebei 050200,China)

机构地区：[1]河北农业大学中兽医学院,河北保定071000 [2]河北维尔利动物药业集团公司,河北石家庄050200 [3]河北省中兽药产业技术研究院,河北石家庄050200 [4]石家庄市禽用中药产业技术研究院,河北石家庄050200 [5]河北省功能性饲料添加剂技术创新中心,河北石家庄050200

出　　处：《中兽医医药杂志》2024年第5期67-73,共7页Journal of Traditional Chinese Veterinary Medicine

基　　金：河北省中兽药产业技术研究院建设补助经费(225790267H);石家庄市禽用中药产业技术研究院(238790749A);河北省功能性饲料添加剂技术创新中心建设补助经费(217790537H);国家“十四五”重点研发计划(2022YFD1801104)。

摘　　要：对满山红配方颗粒的提取工艺进行优化,并建立了相应的质量标准,为其临床应用奠定基础。采用单因素试验结合Box-Behnken响应面法进行研究,以出膏率和杜鹃素含量为评价指标,对提取次数、提取时间和加水倍数进行考察,利用高效液相色谱法(HPLC)对杜鹃素含量进行定量测定,得出各因素对出膏率和杜鹃素含量的影响程度,并依据《中华人民共和国药典》2020年版,采用薄层鉴别法对满山红进行定性分析。通过Box-Behnken中心组和设计模型分析回归模型方差以及响应面图,确认满山红的最佳提取条件为:提取4次,提取时间2 h,加水18倍。薄层鉴别法显示,满山红斑点清晰,重现性佳,高效液相色谱法也显示出高度的准确性和重复性。优化后的满山红配方颗粒提取工艺操作简便、稳定可行,为满山红的深度开发和利用提供了科学依据。The purpose was to optimize the extraction process of Folium Rhododendri Daurici formula granules and establish the corresponding quality standard to lay the foundation for its clinical application.A single-factor test combined with Box-Behnken response surface method was used to investigate the extraction times,extraction time,and water addition times with the evaluation indexes of cream yield and farrerol content,and the farrerol content was quantitatively and accurately determined by high-performance liquid chromatography(HPLC),so as to find out the influences of various factors on the cream yield and farrerol content and qualitatively analyze them according to the pharmacopoeia of Folium Rhododendri Daurici.The results were analyzed by the Box-Behnen method.The optimal extraction conditions of Folium Rhododendri Daurici were confirmed by Box-Behnken central group and design model analysis of the variance of the regression model and the response surface plots:4 extractions for 2 h and 18 times of water.The thin-layer identification method showed clear and reproducible spots of Folium Rhododendri Daurici,and the high-performance liquid chromatography method also showed a high degree of accuracy and reproducibility.The optimized extraction process of Folium Rhododendri Daurici formula granules is not only easy to operate,stable and feasible,but also has a good application prospect,which provides a scientific basis for the deep development and utilization of Folium Rhododendri Daurici.

关 键 词：满山红 杜鹃素 配方颗粒 提取工艺 质量标准 

分 类 号：S853.7[农业科学—临床兽医学]