天智颗粒激活PI3K-AKT信号保护Aβ_(25-35)诱导的PC12细胞凋亡作用研究  

Tianzhi Granules Activated PI3K-AKT Signal to Protect PC12 Cells from Aβ_(25-35)-Induced Apoptosis

作　　者：陈博[1] 程建安[1] 唐镔镔[1] 刘平 郭忠伟[1] CHEN Bo;CHENG Jian'an;TANG Binbin;LIU Ping;GUO Zhongwei(Tongde Hospital of Zhejiang Province,Hangzhou,310012;Southwest Medical University Affiliated Traditional Chinese Medicine Hospital,Luzhou,646099)

机构地区：[1]浙江省立同德医院,浙江杭州310012 [2]西南医科大学附属中医医院,四川泸州646099

出　　处：《浙江中医杂志》2024年第9期757-760,共4页Zhejiang Journal of Traditional Chinese Medicine

基　　金：浙江省基础公益研究计划项目基于静息态功能磁共振对天智颗粒联合多奈哌齐治疗轻中度阿尔茨海默病患者脑功能连接作用机制的研究,编号:LGF21H270003;浙江省医药卫生科技项目基于静息态脑功能连接研究多奈哌齐治疗轻中度阿尔茨海默病内嗅皮层通路的机制,编号:2020KY509;浙江省医坛新秀培养对象,编号:浙卫办〔2021〕40号;四川省科技厅重大科技专项项目重大慢病的中医药诊疗体系构建与示范推广,编号:2022ZDX0022。

摘　　要：目的:研究天智颗粒对Aβ_(25-35)诱导的大鼠嗜铬细胞瘤PC12细胞凋亡的作用及其机制。方法:用不同浓度天智颗粒或天智颗粒+LY294002干预Aβ_(25-35)处理的PC12细胞,通过MTT检测细胞活力,流式细胞仪检测细胞凋亡和氧应激,酶标仪检测Caspase 3活性和丙二醛(MDA)含量,免疫印迹法检测Bcl2/Bax、p-AKT/AKT蛋白水平。结果:天智颗粒剂量依赖地改善Aβ_(25-35)诱导的PC12细胞活力下降,抑制其凋亡,降低Caspase 3活性,上调Bcl2/Bax表达,减少氧应激和MDA的生成,同时促进PI3K/AKT信号的活化。而PI3K抑制剂LY294002干预显著地逆转天智颗粒的这些有益作用。结论:天智颗粒能有效地保护Aβ_(25-35)诱导的PC12细胞凋亡,其机制可能与激活PI3K/AKT信号相关。Objective:To explore the effect and mechanism of Tianzhi granules on Aβ_(25-35)-induced pheochromocytoma PC12 cells apoptosis.Method:PC12 cells were induced by Aβ_(25-35) in the pres⁃ence or absence of different dose of Tianzhi granules without or with LY294002.The cell viability was determined by MTT,apoptosis and oxidative stress were measured by flow cytometry,caspase 3 activity and MDA content were detected by microplate reader.The levels of Bcl2,Bax,p-AKT and AKT protein were determined by western blotting.Results:Tianzhi granules dose-dependently im⁃proved Aβ_(25-35)-induced PC12 cells apoptosis,as indicated by increased cell viability,decreased cas⁃pase 3 activity,as well as upregulating Bcl2/Bax.Furthermore,Tianzhi granules dose-dependently in⁃hibited Aβ_(25-35)-induced oxidative stress and MDA content but activated PI3K/AKT signal pathway,and the inhibitor of PI3K/AKT,LY294002,reversed the beneficial effects of Tianzhi granules in Aβ_(25-35)-primed PC12 cells.Conclusion:Tianzhi granules could effectively protect from Aβ_(25-35)-primed PC12 cells,which might be mediated by activating PI3K/AKT pathway.

关 键 词：天智颗粒 阿尔茨海默病 PC12细胞 凋亡 PI3K-AKT信号 

分 类 号：R285.5[医药卫生—中药学]