桑树NAC基因家族鉴定与生根粉处理下表达模式分析  

Identification of NAC gene family in Morus notabilis and analysis on their expression patterns under rooting powder treatment

作　　者：王艺琳[1] 豆浩 邓鹏 周鑫[1] 权金娥 WANG Yilin;DOU Hao;DENG Peng;ZHOU Xin;QUAN Jin′e(College of Forestry,Henan Agricultural University,Zhengzhou,Henan 450002,China)

机构地区：[1]河南农业大学林学院,河南郑州450002

出　　处：《福建农林大学学报（自然科学版）》2024年第5期629-640,共12页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：河南省林木种质资源调查专项(30601537)。

摘　　要：【目的】探究桑树NAC基因家族的生物信息学特征,并研究1000 mg·L^(-1)生根粉(ABT)处理对该基因产生的影响,为深入研究桑树NAC转录因子的功能提供依据。【方法】利用生物信息学方法对川桑NAC基因家族进行基因鉴定,并分析其理化性质、保守基序、顺式作用元件、系统进化树、蛋白三级结构及互作关系,并选用‘果桑大十’作为试验材料,经ABT处理后,分析其4个时期(10、20、30、40 d)NAC基因家族的表达模式。【结果】桑树NAC基因家族共有92个成员,分为22个亚家族,亚家族成员广泛分布于细胞核,且具有相似的保守结构。顺式作用元件分析表明,桑树NAC基因家族对激素具有较强的响应能力。蛋白三级结构显示同一亚家族的蛋白空间结构相似,且功能相同,这可能与其进化同源性有关。ABT处理后,NAC基因家族的表达水平整体呈上升趋势,而MnNAC91、MnNAC67、MnNAC28在对照组(CK)中呈现较高的表达水平。【结论】NAC基因家族功能相对稳定,在整个进化过程中无明显变化;ABT可促进NAC基因家族表达,尤其是MnNAC75、MnNAC104、MnNAC7、MnNAC30、MnNAC101、MnNAC83、MnNAC102具有较高的同源性,并与拟南芥的结构功能相似,推测对抗干旱胁迫有一定作用。【Objective】The biological information of NAC gene family in Morus notabilis(mulberry tree)were analyzed across the whole genome,and their expression under ABT treatment were investigated to provide a theoretical basis for further research on the function of NAC transcription factors in mulberry trees.【Method】The NAC gene family were identified via whole-genome sequencing,followed by analysis on physical and chemical properties,conserved motifs,cisacting elements,phylogenetic tree,protein tertiary structure,and interaction association by bioinformatics methods.Furthermore,the expression patterns of the NAC gene family in Fruit Mulberry Big Ten cultivar were analyzed under the treatment of 1000 mg·L^(-1) ABT,as well as pure water as the control.【Result】The mulberry NAC gene family has 92 members,divided into 22 subfamilies.Subfamily members are widely distributed in the nucleus of cells,with similar conserved structures.Cisacting element analysis showed higher influence of NAC gene family on hormone response,and the proteins in the same subfamily were similar in tertiary structure and function,which may be related to evolutionary homology.After treatment with 1000 mg·L^(-1) ABT,the expression of most MnNAC family members showed an uprising trend,conversely,those of MnNAC91,MnNAC67 and MnNAC28 were higher in the control.【Conclusion】The functions of NAC gene family were relatively stable,without significant variation throughout the evolutionary process.ABT can promote the expression of NAC genes.MnNAC75,MnNAC104,MnNAC7,MnNAC30,MnNAC101,MnNAC83,and MnNAC102 had high homology and similar structural functions as Arabidopsis,implying their responses to drought stress.

关 键 词：桑树 NAC基因家族 鉴定分析 生物信息 元件分析 

分 类 号：S723[农业科学—林木遗传育种]