利用流式细胞术鉴定3种李属植物基因组大小及染色体倍性检测  

Identification of genome size and chromosomal ploidy of three prunus species by flow cytometry

作　　者：王亚铜 郭婧婧 杨芬芬 牛莹莹 章世奎[2] 樊国全[2] 王尚栋 耿文娟[1] WANG Yatong;GUO Jingjing;YANG Fenfen;NIU Yingying;ZHANG Shikui;FAN Guoquan;WANG Shangdong;GENG Wenjuan(College of Horticulture,Xinjiang Agricultural University,Urumqi 830052,China;Luntai Fruit Tree Germplasm Resource Nursery,Xinjiang Academy of Agricultural Sciences,Luntai Xinjiang 841600,China)

机构地区：[1]新疆农业大学园艺学院,乌鲁木齐830052 [2]新疆农业科学院轮台果树资源圃,新疆轮台841600

出　　处：《新疆农业科学》2024年第7期1673-1681,共9页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区高校基本科研业务费科研项目(XJEDU2022Z005)。

摘　　要：【目的】研究适合不同李属资源的FCM检测体系,估测其基因组大小及倍性,为李属资源的鉴定、分类及基因组和转录组等深入研究提供依据。【方法】利用FCM对3种常用的细胞核裂解液进行筛选与优化,选取最适宜的裂解液鉴定3种李属植物进行基因组大小和检测染色体倍性。【结果】LB01、Marie’s和WPB 3种裂解液对樱桃李检测效果均较好,而WPB裂解液检测效果最佳;Marie’s和LB01裂解液与欧洲李不匹配,改良的WPB裂解液最适合欧洲李;杏李与LB01裂解液不匹配,WPB裂解液对杏李的裂解效果好于Marie’s裂解液,而改良的WPB裂解液对杏李的裂解效果均最好。樱桃李和杏李基因组大小范围分别为491.22~566.40 Mb和514.46~573.83 Mb,欧洲李资源平均基因组大小为1678.37 Mb。【结论】樱桃李和杏李资源均为二倍体,欧洲李资源全部为六倍体,李属植物每个种内不同品种之间的基因组大小存在一定差异。【Objective】To explore FCM detection system suitable for different Prunus resources,estimate their genome size and ploidy,and provide basis for the identification,classification,genome and transcriptome of Prunus resources.【Methods】Three commonly used nuclear lysates were screened and optimized by FCM,and the most suitable lysates were selected to detect the genome size and chromosome ploidy of three plum plants.【Results】LB01,Marie's and WPB lysates were all effective in the detection of P.simonii Carr.,and WPB lysates was the best.The two lysates Marie's and LB01 did not match with P.domestica L.,and the improved WPB lysate was the most suitable for P.simonii Carr.and LB01 lysate did not match,WPB lysate had better cracking effect on P.simonii Carr.than Marie's lysate,and the improved WPB lysate had the best cracking effect on P.simonii Carr.The two lysates Marie's and LB01 did not match with P.domestica L.,and the improved WPB lysate was the most suitable for P.domestica L..The P.simonii Carr.and LB01 lysate did not match,WPB lysate had better cracking effect on P.simonii Carr..than Marie's lysate,and the improved WPB lysate had the best cracking effect on P.simonii Carr.With genome sizes ranging from 491.22 to 566.40 Mb and 514.46 to 573.83 Mb,respectively,with an average genome size of 1,678.37 Mb.【Conclusion】The identified germplasm resources of P.cerasifera Ehrh.and P.simonii Carr.were all diploid,while the P.domestica L.germplasm resources were all hexaploid,the genome size of different varieties in each species of Prunus genus is different.

关 键 词：李 流式细胞术(FCM) 染色体倍性 基因组大小 

分 类 号：S188[农业科学—农业基础科学]