一株海洋杆菌属新菌种XAAS-72^(T)的植物促生功能分析及ACC脱氨酶蛋白结构预测  

Structure prediction of the ACC protein from Pontibacter kalidii XAAS-72^(T) with the plant growth-promoting character

作　　者：王慧楠 朱静[2] 谢文文 何子璇 柏晓玉 朱艳蕾[1] 张志东 WANG Huinan;ZHU Jing;XIE Wenwen;HE Zixuan;BAI Xiaoyu;ZHU Yanlei;ZHANG Zhidong(College of Life Sciences,Xinjiang Normal University,Urumqi 830054,China;Xinjiang Key Laboratory of Special Environmental Microbiology/Institute of Applied Microbiology,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China;College of Life Sciences and Technology,Xinjiang University,Urumqi 830046,China)

机构地区：[1]新疆师范大学生命科学学院,乌鲁木齐8300541 [2]新疆农业科学院微生物应用研究所/新疆特殊环境微生物实验室,乌鲁木齐830091 [3]新疆大学生命科学与技术学院,乌鲁木齐830046

出　　处：《新疆农业科学》2024年第7期1778-1785,共8页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区自然科学基金项目“杰出青年基金”(2022D01E19);新疆农业科学院科技创新重点培育专项(xjkcpy-2022004);新疆农业科学院农业科技创新稳定支持专项(xjnkywdzc-2023005)。

摘　　要：【目的】研究海洋杆菌属新菌种XAAS-72的植物促生功能,挖掘其潜在功能基因。【方法】通过对菌株全基因组测序,分析相关功能基因组成,挖掘ACC脱氨酶合成相关的候选基因,并进行功能预测。【结果】海洋杆菌属新种Pontibacter kalidii XAAS-72菌悬液处理可显著提高盆栽小麦麦苗生长,其基因组长度为5054860 bp,含1个环形质粒,总GC含量为54.52%,注释的基因数目为4391个,编码蛋白数4261个,具有多种抗逆和促生相关基因。其与Pontibacter sp.BAB1700的ACC脱氨酶(1-aminoeyclopropane-1-earboxylate-deaminase)相似度最高为72.48%。该蛋白属于不稳定亲水性蛋白,不具备跨膜结构,且无信号肽结构。【结论】海洋杆菌属新种XAAS-72蕴藏着丰富的抗逆和植物促生相关基因。【Objective】To explore the plant growth promotion function of a novel strain Pontibacter kalidii XAAS-72 T,and investigate the potential functional genes.【Methods】The strain whole genome was sequenced,and the composition of function genes were analyzed.A candidate gene related to ACC deaminase synthesis was obtained,and the enzyme protein characteristics were predicted.【Results】The results showed that the growth of wheat seed treated with Pontibacter kalidii XAAS-72 T was promoted significantly in pots.The genome length of strain XAAS-72(accession no.CP111079)was 5,054,860 bp,containing one circular plasmid.A total GC content was 54.52%,and the number of annotated genes was 4,391,coding for 4,261 proteins.A variety of resistance and growth-promoting related genes was observed.It had the highest similarity of 72.48%to the ACC deaminase of Pontibacter sp.BAB1700.It an unstable hydrophilic protein,with none of transmembrane structure and signal peptide structure.【Conclusion】Pontibacter kalidii XAAS-72 T harbors a lot of genes related to the stress resistance and the plant growth-promoting.

关 键 词：海洋杆菌属 植物促生特性 ACC脱氨酶 结构预测 

分 类 号：S188[农业科学—农业基础科学]