丹参酮ⅡA对胃癌BGC-823细胞增殖及凋亡的影响  

Effect of TanshinoneⅡA on Viability and Apoptosis of Human Gastric Cancer BGC-823 Cells

作　　者：杜婧雯 何奇 丁思嫄 王馨 方贵海 张巍 DU Jingwen;HE Qi;DING Siyuan;WANG Xin;FANG Guihai;ZHANG Wei(Jilin Medical University School of Clinical Medicine,Jilin Province 132013;Jilin Medical University School of Public Health,Jilin Province 132013;Jilin Medical University School of Basic Medicine,Jilin City,Jilin Province 132013;School of Basic Medi-cine,Beihua university,Jilin City,Jilin Province 132013,China)

机构地区：[1]吉林医药学院临床医学院,吉林吉林132013 [2]吉林医药学院公共卫生学院,吉林吉林132013 [3]吉林医药学院基础医学院,吉林吉林132013 [4]北华大学基础医学院,吉林吉林132013

出　　处：《吉林医药学院学报》2024年第5期335-338,共4页Journal of Jilin Medical University

基　　金：吉林省科技厅科技发展项目(20190701062GH);吉林省教育厅“十三五”科学技术研究项目(JJKH20200453KJ);吉林医药学院2022年度大学生创新创业训练计划项目(X202213706003)。

摘　　要：目的以胃癌BGC-823细胞为研究对象,观察不同浓度丹参酮ⅡA对胃癌BGC-823细胞活力的影响,探讨丹参酮ⅡA促BGC-823细胞凋亡机制。方法取对数生长期的BGC-823细胞,分别加入0.5、1.0、2.0、5.0 mg/L浓度丹参酮ⅡA培养12 h。光学显微镜观察BGC-823细胞形态学变化,MTT实验检测丹参酮ⅡA对细胞活力的影响,流式细胞术检测细胞凋亡率,Western blot实验检测凋亡相关蛋白BcL-2,Bax和Cleaved-Caspase-3的表达。结果光学显微镜观察发现,不同浓度丹参酮ⅡA均可导致细胞形态发生改变;MTT实验显示,丹参酮ⅡA作用于BGC-823细胞12h后,明显降低细胞活力,并且存在剂量依赖性(P<0.05或P<0.01);流式细胞术检测结果显示,丹参酮ⅡA对BGC-823细胞作用12 h后各浓度组早期凋亡比例分别为(5.80±0.32)%(P<0.05)、(7.90±0.43)%(P<0.05)、(10.20±0.42)%(P<0.01)、(20.44±1.24)%(P<0.01);Western blot实验检测结果显示,不同浓度丹参酮ⅡA作用细胞12 h后,与对照组相比,丹参酮ⅡA实验组BGC-823细胞Bax和Cleaved-Caspase-3蛋白表达水平呈上调趋势,Bcl-2蛋白表达水平呈下调趋势。结论丹参酮ⅡA抑制BGC-823细胞活力并促进凋亡,且具有剂量依赖性。Objective In this study,the effects of different concentrations of tanshinoneⅡA on the viability of gastric cancer BGC-823 cells were observed,and the mechanism of tanshinoneⅡA promoting the apoptosis of BGC-823 cells was investigated.Methods BGC-823 cells at logarithmic growth stage were cultured with 0.5,1.0,2.0 and 5.0 mg/L tanshinoneⅡA,respectively,for 12 hours.The mor⁃phological changes of BGC-823 cells were observed by optical microscope,and the inhibitory effect of tanshinoneⅡA on cell viability was detected by MTT assay.Flow cytometry was used to detect apoptosis rate.The expressions of apoptosis-related proteins Bcl-2,Bax and Cleaved-Caspase-3 were detected by Western blot.Results Light microscopy showed that different concentrations of tanshinoneⅡA could lead to changes in cell morphology.The MTT assay demonstrated a significant inhibition of BGC-823 cell viability following 12-hour treatment with tanshinoneⅡA in a dose-dependent manner(P<0.05 or P<0.01).Flow cytometry demonstrated that following a 12-hour treatment of tanshinoneⅡA on BGC-823 cells,the proportions of early apoptosis in each concentration group were(5.80±0.32)%(P<0.05),(7.90±0.43)%(P<0.05),(10.20±0.42)%(P<0.01),and(20.44±1.24)%(P<0.01),respectively.The results of the Western blot assay demonstrated that following the treatment of BGC-823 cells with various concentrations of tanshinoneⅡA for a duration of 12 hours,there was a tendency for the expression levels of Bax and Cleaved Caspase-3 protein in the experimental group to be up-regulated,in comparison to the control group.Similarly,the expression levels of Bcl-2 protein appeared to be down-regulated.Conclusions Tan⁃shinoneⅡA inhibits BGC-823 cell viability and promotes apoptosis in a dose-dependent manner.

关 键 词：丹参酮ⅡA 细胞活力 细胞凋亡 BGC-823细胞 

分 类 号：R273[医药卫生—中西医结合]