外源赤霉素对干旱胁迫下中华常春藤生理特性的影响  

Effects of Exogenous Gibberellic Acid on Physiological Characteristics of Hedera nepalensis var.sinensis under Drought Stress

作　　者：胡纪龙 崔仕方 闫钘 许红连 黄晓霞[1] HU Jilong;CUI Shifang;YAN Xing;XU Honglian;HUANG Xiaoxia(College of Landscape and Horticulture,Southwest Forestry University,Kunming 650224,China)

机构地区：[1]西南林业大学园林园艺学院,云南昆明650224

出　　处：《云南农业大学学报（自然科学版）》2024年第4期110-118,共9页Journal of Yunnan Agricultural University:Natural Science

基　　金：云南省高层次人才“青年拔尖人才”(YNWR-QNBJ-2020-222)。

摘　　要：【目的】探究外源赤霉素(gibberellic acid,GA_(3))对干旱胁迫下中华常春藤生理特性的影响。【方法】以中华常春藤扦插苗为材料进行良好水分处理与干旱胁迫处理(10%PEG-6000),并喷施不同质量浓度(0、50和100 mg/L)的外源GA_(3),处理15 d后,测定植物叶片的光合色素含量、气体交换参数、叶绿素荧光参数、丙二醛含量、渗透调节物质含量及抗氧化酶活性。【结果】与良好水分处理组相比,干旱胁迫下中华常春藤叶片的光合色素含量和叶绿素荧光参数均显著下降;丙二醛、脯氨酸、可溶性蛋白和可溶性糖含量以及抗氧化酶活性均显著上升。通过外源喷施50和100 mg/L GA_(3)溶液,中华常春藤的光合色素含量、最大光化学效率、实际光化学效率和抗氧化酶活性都得到了显著提升。【结论】外源喷施50和100 mg/L GA_(3)能够缓解干旱胁迫对中华常春藤的伤害,提高其抗旱性,且100 mg/L GA_(3)效果更好。[Purpose]To investigate the effects of exogenous gibberellic acid(GA_(3))on the physiological characteristics of Hedera nepalensis var.sinensis under drought stress.[Methods]H.nepalensis var.sinensis cuttings were treated with well-watered and drought stress(10%PEG-6000)as well as sprayed with different mass concentrations of exogenous GA_(3)(0,50 and 100 mg/L).The content of photosynthetic pigments,gas-exchange parameters,chlorophyll fluorescence parameters,malondialdehyde(MDA)content,osmotic regulated substances contents and antioxidant enzyme activities of leaves under different treatments were determined after 15 days.[Results]Compared with the well-watered group,the content of photosynthetic pigments and chlorophyll fluorescence parameters of leaves under drought stress decreased significantly;while the contents of MDA,proline,soluble protein,soluble sugar and antioxidant enzyme activities increased significantly.By exogenous spraying of GA_(3) with 50 and 100 mg/L,the photosynthetic pigment content,maximum quantum efficiency,quantum efficiency value and antioxidant enzyme activities were significantly improved.[Conclusion]Spraying exogenous GA_(3) is able to alleviate the damage of drought stress on H.nepalensis var.sinensis and improve its drought resistance.

关 键 词：外源赤霉素 中华常春藤 干旱胁迫 生理特性 

分 类 号：S687.301[农业科学—观赏园艺]