35份水稻骨干亲本氮高效基因分析及功能分子标记开发  

Analysis of High Nitrogen Utilization Efficiency Genes and Development of Functional Molecular Markers in 35 Rice Backbone Parents

作　　者：龙武华 吴朝昕 刘雪薇 吴娴 姜雪 朱速松[1,2] LONG Wuhua;WU Chaoxin;LIU Xuewei;WU Xian;JIANG Xue;ZHU Susong(Rice Research Institute,Guizhou Academy of Agricultural Sciences,Guiyang Guizhou 550006;Key Laboratory of Crop Genetic Resources and Germplasm Innovation in Karst Region,Ministry of Agriculture and Rural Affairs,Guiyang Guizhou 550006)

机构地区：[1]贵州省农业科学院水稻研究所,贵州贵阳550006 [2]农业农村部喀斯特山区作物基因资源与种质创新重点实验室,贵州贵阳550006

出　　处：《现代农业科技》2024年第17期163-167,共5页Modern Agricultural Science and Technology

基　　金：贵州省科技计划项目“优质、多抗、高产、高效粳稻新种质创制”(黔科合平台人才〔2018〕5263);贵州省科技计划项目“农作物种质资源精准鉴定评价(优异基因挖掘与利用)”(黔科合支撑〔2022〕重点026);贵州省科技计划项目“贵州山区特色优质稻新品种选育”(黔科合支撑〔2022〕重点028)。

摘　　要：氮是影响水稻产量形成最重要的元素之一,其合理和高效利用是农业可持续发展的重要保障。通过靶向测序技术鉴定了35份水稻骨干亲本OsNPF6.1、NRT1.1B、OsNR2和OsGRF4的基因型,并基于PARMS技术(penta-primer amplification refractory mutation system)开发了OsNPF6.1、OsNR2和OsGRF4的荧光功能分子标记。结果表明,荧光功能分子标记检测结果与靶向测序结果一致,并且明确了靶向测序未明确的氮高效基因型。综上所述,开发的氮高效基因功能分子标记为培育氮高效水稻新品种提供了技术支撑。Nitrogen is one of the most important elements affecting rice yield formation,and its rational and efficient utilization is an important guarantee for sustainable development of agriculture.The genotypes of four genes(OsNPF6.1,NRT1.1B,OsNR2 and OsGRF4)in 35 rice backbone parents were identified through targeted sequencing technology,and fluorescent functional molecular markers for OsNPF6.1,OsNR2 and OsGRF4 were developed based on PARMS technology(penta-prime amplification refractory mutation system).The results showed that the fluorescent functional molecular marker detection results were consistent with the targeted sequencing results,and identified high nitrogen utilization efficiency genotypes that were not clearly identified by targeted sequencing.In summary,the developed high nitrogen utilization efficiency gene functional molecular markers provided technical support for cultivating new rice varieties with high nitrogen utilization efficiency.

关 键 词：水稻 骨干亲本 氮高效基因 靶向测序 分子标记 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]