新型冠状病毒多种Omicron亚变异株利用TMEM106B感染宿主细胞特性的研究  

Characterization of infections by various SARS-CoV-2 Omicron subvariants mediated by TMEM106B receptor

作　　者：王媛媛[1] 陈丹瑛 刘雨欣 宋焱君 孙慧 张媛媛[2] 赵学森[1] Wang Yuanyuan;Chen DanyingLiu Yuxin;Song Yanjun;Sun Hui;Zhang Yuanyuan;Zhao Xuesen(Peking University Ditan Teaching Hospital,Beijing 100015,China;Institute of Infectious Diseases,Beijing Ditan Hospital,Capital Medical University,Beijing 100015,China)

机构地区：[1]北京大学地坛医院教学医院,100015 [2]首都医科大学附属北京地坛医院传染病研究所,100015

出　　处：《国际病毒学杂志》2024年第4期291-295,共5页International Journal of Virology

基　　金：国家重点研发计划(2023YFC0872400);北京市自然科学基金(M23005)。

摘　　要：目的研究跨膜蛋白106B(transmembrane protein 106B,TMEM106B)支持新型冠状病毒(severe acute respiratory syndrome coronavirus 2,SARS-CoV-2)多种Omicron亚变异株感染侵入的特性。方法构建TMEM106B-knockout Flp-In T-Rex 293细胞系(TMEM106BKO),利用假病毒感染系统以及GFP1-10和GFP11的双荧光互补系统的合胞体形成实验检测TMEM106B在SARS-CoV-2多种Omicron亚变异株刺突蛋白(spike protein,S)介导的侵入和细胞-细胞融合中发挥的作用。结果SARS-CoV-2多种Omicron亚变异株对TMEM106B-knockout Flp-In T-Rex 293细胞的侵入效率存在不同程度的降低,并且TMEM106B可促进SARS-CoV-2多种Omicron亚变异株S蛋白介导的细胞-细胞融合。结论TMEM106B可作为受体分子支持SARS-CoV-2多种Omicron亚变异株的侵入和细胞-细胞融合,为治疗新型冠状病毒感染提供新的思路。ObjectiveTo investigate the role of transmembrane protein 106B(TMEM106B)in supporting the entry of multiple Omicron subvariants of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).MethodsTMEM106B-knockout Flp-In T-Rex293 cell line(TMEM106B KO)was constructed.The pseudoviral infection systems and the syncytia formation assay based on dual fluorescent complementary system of GFP1-10 and GFP11 were established to detect the role of TMEM106B in spike protein-mediated entry and cell-cell fusion during the infections by different SARS-CoV-2 Omicron subvariants.ResultsThe SARS-CoV-2 Omicron subvariants showed varying degrees of reduced efficiency in infecting TMEM106B KO,and TMEM106B can facilitate cell-cell fusion mediated by spike protein of various SARS-CoV-2 Omicron subvariants.ConclusionsTMEM106B acted as a receptor to support the entry and cell-cell fusion of multiple Omicron subvariants of SARS-CoV-2.It could be a new idea for the treatment of SARS-CoV-2 infection.

关 键 词：跨膜蛋白106B 新型冠状病毒 Omicron亚变异株 

分 类 号：R511[医药卫生—内科学]