两种方法在城市污水新型冠状病毒与多种腹泻病毒检测中的差异分析  

Differences between two methods in detection of SARS-CoV-2 and diarrhea viruses in sewage

作　　者：杜阳光[1] 王潇 耿洁 魏丹 童晶[1] Du Yangguang;Wang Xiao;Geng Jie;Wei Dan;Tong Jing(Microbiology Laboratory Department,Xuzhou Center For Disease Prevention and Control,Xuzhou 221006,China)

机构地区：[1]江苏省徐州市疾病预防控制中心微生物检验科,221006

出　　处：《国际病毒学杂志》2024年第4期301-305,共5页International Journal of Virology

基　　金：江苏省卫生健康委科研课题(Y12023007)。

摘　　要：目的研究两种浓缩方法在新型冠状病毒与多种腹泻病毒核酸检测中的差别,了解污水中不同病毒的流行趋势,为防控方案的制定提供理论依据。方法使用聚乙二醇沉淀法和阴离子膜法对污水中新型冠状病毒、诺如病毒、轮状病毒、札如病毒、腺病毒、人类星状病毒进行富集浓缩和核酸检测,对其结果进行分析。结果腺病毒阴离子膜法浓缩效果优于聚乙二醇沉淀法,诺如病毒、轮状病毒和札如病毒聚乙二醇沉淀法效果更好,人类星状病毒两种浓缩效果没有统计学差异。新型冠状病毒检出率随时间有波动,3月2日以后聚乙二醇沉淀法检出的ORF1ab基因和N基因的浓缩效果优于阴离子膜法。结论两种方法对污水中不同病毒的富集浓缩效果不同,可根据工作需要选用合适的病毒浓缩方法。ObjectiveTo evaluate the differences between two enrichment methods in detection of nucleic acids from severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)and various diarrheal viruses and access the epidemiological trends of the viruses in sewage water,so as to provide theoretical support for the formulation of prevention and control plans.MethodsThe polyethylene glycol precipitation method and anions membrane method ultrafiltration method were used to concentrate and detect SARS-CoV-2,norovirus(Nov),rotavirus(RV),sapovirus(SaV),adenovirus(AdV)and human astrovirus(HAstV)in sewage and the results were analyzed.ResultsThe anions membrane method ultrafiltration method was better than the polyethylene glycol precipitation method for concentration of AdV.The polyethylene glycol precipitation method had higher concentration efficiency for Nov,RV and SaV.There is no statistically significant difference between the two methods for enriching of HAstV.The detection rate of the SARS-CoV-2 varied over time.After March 2,the concentration effect for Orf1ab and N genes by polyethylene glycol precipitation method was better than that of anion membrane method.ConclusionsThe two methods showed different concentration effects for different viruses in sewage and should be selected according to the needs of the work.

关 键 词：污水 富集浓缩 新型冠状病毒 腹泻病毒 

分 类 号：R115[医药卫生—公共卫生与预防医学]