2007—2019年北京市朝阳区甲型H3N2流感病毒HA基因特征分析  

Genetic characteristics of HA gene of influenza A(H3N2)virus in Chaoyang district of Beijing from 2007 to 2019

作　　者：孙灵利[1] 刘丹[1] 高艳[1] 赵剑虹[1] 张玉松[1] 韩桃利 Sun Lingli;Liu Dan;Gao Yan;Zhao Jianhong;Zhang Yusong;Han Taoli(Microbiological Laboratory,Beijing Chaoyang Center for Disease Control and Prevention,Beijing 100021,China)

机构地区：[1]北京市朝阳区疾病预防控制中心微生物检验科,100021

出　　处：《国际病毒学杂志》2024年第4期316-321,共6页International Journal of Virology

基　　金：北京市朝阳区科技计划项目(CYSF2050);北京市朝阳区科技计划项目(CYSF2206);首都卫生发展科研专项项目(2022-1G-4231)。

摘　　要：目的了解北京市朝阳区甲型H3N2亚型流感病毒HA基因变异情况,为评估疫苗有效性提供基础数据。方法从全球流感共享数据库(GISAID)下载2007—2019年世界卫生组织推荐流感疫苗株及各分支代表株序列,与本研究H3N2亚型分离株的HA基因序列进行系统进化、氨基酸变异分析和疫苗效力评估。结果2015年及之前的H3N2亚型流感多数属于3C.3a分支,2016年开始以3C.2a分支为主,2017年以3C.2a2为主,并进化至3C.2a3,2019年又转换为3C.3a1亚分支;2009年、2013年和2015年存在3C.2和3C.3分支共流行的现象。抗原决定簇A~D区共计存在54个氨基酸变异位点;受体结合部位的130环、190螺旋和220环区域均有氨基酸位点发生变异;各年均有发生糖基化位点的缺失/增加/转换,多数发生在HA1区域。预测流感疫苗效力(VE)为5.00%~47.00%(PepitopeMax值:0~0.2105)。结论北京市朝阳区2007—2019年H3N2流感病毒HA基因进化具有多样性和复杂性,且有可能发生了抗原漂移,部分年份流行株与推荐疫苗株匹配性较差,提示持续监测H3N2亚型流感病毒HA基因变异的必要性。ObjectiveTo explore the genetic variation of the HA gene of influenza A(H3N2)virus in Chaoyang district of Beijing,so as to provide basic data for the evaluation of the vaccine efficacy.MethodsThe sequences of recommended influenza vaccine strains and representative strains by World Health Organization from 2007 to 2019 were downloaded from the Global Initiative on Sharing All Influenza Data(GISAID),and were used for the phylogenetic analysis,amino acid variation analysis and the evaluation of vaccine efficacy.with the HA gene sequence of influenza A(H3N2)virus strains in the present study.ResultsMost influenza A(H3N2)virus strains in and before 2015 belonged to 3C.3a branch,and strains in 3C.2a branch started to be dominant from 2016.In 2017,the dominant strains were in 3C.2a2 sub-family and evolved to 3C.2a3.In 2019 the dominant strains returned to 3C.3a1 sub-family.In 2009,2013 and 2015,there existed co-circulation of strains in 3C.2 and 3C.3 branches.There were 54 amino acid variation sites in the epitopes A to D in HA gene.Amino acid mutations occurred in the 130 loop region,190 helix region and 220-ring region.The deletion/addition/conversion of glycosylation sites occurred every year,mostly in the HA1 region.The vaccine efficacy was predicted to be 5%-47%(Pepitope Max:0-0.2105).ConclusionsThe evolution of HA gene of of influenza A(H3N2)virus in Chaoyang district of Beijing from 2007 to 2019 showed diversity and complexity with possible antigen drift.The matching of epidemic strains with recommended vaccine strains were poor in some years,suggesting the necessarity of continuous surveillance the HA gene variation of influenza A(H3N2)virus.

关 键 词：甲型H3N2流感病毒 表面糖蛋白基因 血凝素 氨基酸变异 疫苗效力 

分 类 号：R181.3[医药卫生—流行病学] R511.7[医药卫生—公共卫生与预防医学]