云南省2022年手足口病病例中柯萨奇病毒B5型VP1区基因特征分析  

Genetic analysis on VP1 gene of coxsackievirus B5 isolated from HFMD cases in Yunnan province,2022

作　　者：罗定富 姜黎黎[2] 寸建萍[2] 杨溪[2] 周晓芳[2] 曹亿会[2] 伏晓庆[2] 郭天巧 田炳均[2] Luo Dingfu;Jiang Lili;Cun Jianping;Yang Xi;Zhou Xiaofang;Cao Yihui;Fu Xiaoqing;Guo Tianqiao;Tian Bingjun(Laboratory Center of Dehong Center for Disease Control and Prevention,Mangshi 678400,China;Institute of Acute Infectious Diseases,Yunnan Center for Disease Control and Prevention,Kunming 650022,China)

机构地区：[1]云南省德宏州疾病预防控制中心中心检验室,芒市678400 [2]云南省疾病预防控制中心急性传染病控制科,昆明650022

出　　处：《国际病毒学杂志》2024年第4期322-326,共5页International Journal of Virology

基　　金：国家"十三五"艾滋病和病毒性肝炎等重大传染病防治科技重大专项(2017ZX10103010)。

摘　　要：目的对云南省2022年从手足口病(hand,foot and mouth disease,HFMD)病例中分离到的7株CVB5病毒进行分子生物学鉴定并对它们的VP1区基因特征进行分析。方法对2022年云南省手足口病实验室监测网络收集到的病例粪便标本进行病毒分离,对阳性标本先进行VP4/VP2结合区逆转录聚合酶链反应(reverse transcription polymerase chain reaction,RT-PCR)和基因测序分型,再对VP1区进行RT-PCR和测序,用MEGA 7.0软件构建病毒VP1区基因进化树,进行基因特征分析。结果2022年云南省共从HFMD病例粪便标本中检测到7株CVB5病毒,基于VP1区基因序列同源性分析表明,该7株病毒最接近2010年分离于河南省1株CVB5病毒COXB5-Henan-2010,7株病毒之间的核苷酸相似性为98.00%~99.65%,氨基酸同源性为99.30%~100%;7株病毒与河南株的核苷酸相似性为87.36%~88.68%,氨基酸同源性为82.96%~84.85%。基因进化分析表明,CVB5可分为4个基因型(基因型A~D),云南分离株属于基因型C并单独成为一个进化分支。4个基因型之间的核苷酸差异率为18.90~22.30%,大于75%,符合肠道病毒基因分型惯例。结论云南省手足口病实验室检测网络2022年共检测到7株CVB5,虽然数量不多,但证明该病毒是HFMD的病原体之一并在云南省HFMD病例中存在,且单独为一个进化分支。应进一步加强对非EV-A71和非CVA16等其他肠道病毒尤其是CVB5的检测和分子流行病学分析。ObjectiveTo perform molecular typing of 7 strains of coxsackievirus B5(CVB5)isolated from hand,foot and mouth disease(HFMD)cases in Yunnan province in 2022 and analyze the genetic characteristics of the VP1 gene.MethodsThe stool samples from HFMD cases through HFMD laboratory surveillance system of Yunnan province in 2022 were used for virus isolation.For positive samples,reverse transcription polymerase chain reaction(RT-PCR)and sequencing were used for VP4/VP2 region first,and then for VP1 gene.The phylogentic tree of VP1 gene was constructed using MEGA 7.0 software for the genetic analysis.ResultsSeven CVB5 strains were detected from the stool samples of HFMD patients in Yunnan province,2022.The similarity analysis based on VP1 gene sequences showed that the 7 CVB5 strains were most closed to the Henan strain COXB5-Henan-2010.The nucleotide(nt)and amino acid(aa)similarities among the 7 strains were 98.00%-99.65%and 99.30%-100%,respectively.The nt and aa similarities between the 7 strains and Henan strain were 87.36%-88.68%and 82.96%-84.85%,respectively.The phylogentic analysis showed that CVB5 could be divided into 4 genotypes(genotype A-D).The Yunnan strains belonged to genotype C and clustered into a sole lineage.The nt diversities among 4 genotypes was 18.90%-22.30%which were more than 75%in similarity and met the requirement of international enteroviruses(EVs)genotype assignment.ConclusionsSeven CVB5 strains were identified by HFMD surveillance system in Yunnan province in 2022.Although the number was relatively lower,CVB5 was proved to be one of the causative pathogens of HFMD.The virus did exist in Yunnan province and clustered into a sole lineage.Etiological surveillance and molecular analysis for EVs other than EV-A71 and CVA16,especially CVB5 should be enhanced.

关 键 词：手足口病 肠道病毒 柯萨奇病毒B组5型 VP1基因 基因特征分析 

分 类 号：R725.1[医药卫生—儿科] R181.3[医药卫生—临床医学]