一贯煎肾毒性的气质联用代谢组学研究  

Metabolomics of Yiguan Decoction Nephrotoxicity by GC-MS

作　　者：徐瑞平 缪潇瑶 肖炳坤 杨建云 李志恒 黄荣清 XU Rui-ping;MIAO Xiao-yao;XIAO Bing-kun;YANG Jian-yun;LI Zhi-heng;HUANG Rong-qing(College of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 511436,China;Institute of Radiation Medicine,Academy of Military Medical Sciences,Academy of Military Sciences,Beijing 100850,China)

机构地区：[1]广东药科大学中药学院,广州511436 [2]军事科学院军事医学研究院辐射医学研究所,北京100850

出　　处：《科学技术与工程》2024年第24期10199-10205,共7页Science Technology and Engineering

基　　金：国家自然科学基金(21375147)。

摘　　要：应用气相色谱-质谱联用(gas chromatography-mass spectrometer, GC-MS)代谢组学方法考察一贯煎对大鼠肾毒性作用并探索其相关作用通路及作用机制。设置空白组、模型组、以及一贯煎高、中、低剂量的给药组,大鼠连续灌胃21 d。采用GC-MS技术分析各组大鼠血清,获取差异性代谢物信息;采用主成分分析(principal component analysis, PCA)对空白组和模型组代谢物分类,寻找并分析一贯煎干预大鼠后血清中差异性代谢物及含量变化。结果表明:一贯煎高、中、低剂量组的大鼠肾脏病理切片均见不同程度损伤;高剂量组大鼠代谢轮廓趋近于模型组;应用GC-MS确定血清中16种差异代谢物。一贯煎涉及的3条主要代谢通路为:苯丙氨酸、酪氨酸和色氨酸生物合成,硫代谢,甘油酯代谢。To investigate the nephrotoxic effect and related pathway evaluation of Yiguan Decoction on rats using gas chromatography-mass spectrometry(GC-MS)metabolomics method.A blank group,a model group,and a consistently high,medium,and low dose administration group were set up,with rats continuously gavaged for 21 days.GC-MS technology was used to analyze the serum of rats in each group and obtain differential metabolite information.Principal component analysis(PCA)was used to classify the metabolites in the blank group and model group,and the differential metabolites and changes in content in the serum of rats with Yiguan Decoction intervention were searched and analyzed.The results show that pathological sections of the kidneys of rats in the Yiguan Decoction high,medium,and low dose groups show varying degrees of damage.The metabolic profile of the high-dose group rats approach that of the model group.16 differential metabolites in serum were identified.The three main metabolic pathways involved in the Yiguan Decoction are:biosynthesis of phenylalanine,tyrosine,and tryptophan,sulfur metabolism,and glyceride metabolism.

关 键 词：一贯煎 肾毒性 气相色谱-质谱联用(GC-MS) 代谢组学 

分 类 号：R917[医药卫生—药物分析学]